2010 Fiscal Year Final Research Report
A model of granuloma formation induced by hyperactivation of NOD2 signaling
| Project/Area Number |
21791089
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
KANAZAWA Nobuo Wakayama Medical University, 医学部, 講師 (90343227)
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| Project Period (FY) |
2009 – 2010
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| Keywords | NOD2 / 肉芽腫 / NF-κB |
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| Research Abstract |
Human monocytic THP-1 cells expressing early-onset sarcoidosis/Blau syndrome-associated mutant NOD2 were applied for analysis of their phenotype without stimulation or when stimulated with PMA, which induces their differentiation into macrophage-like cells. No significant difference was observed among phenotype of THP-1 derivatives without stimulation. After PMA addition, mutant THP-1 cells were attached to the culture plate for a long period without proliferation, while control cells were attached only transiently. By analysis of surface adhesion molecules, surface ICAM-1 expression level was shown to be correlated with capacity of the cells to attach the plate. Furthermore, PDGF-B was discovered as a cytokine which is specifically produced by mutant THP-1 cells after PMA stimulation. Actually, it was confirmed that ICAM-1 and PDGF-B were expressed on mutant Nod2-expressing multinucleated giant cells in the granulomatous skin lesion of an early-onset sarcoidosis patient.
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[Journal Article] Preliminary study of etidronate for prevention of corticosteroid-induced osteoporosis due to oral glucocorticoid therapy.2011
Author(s)
Furukawa F, Kaminaka C, Ikeda T, Kanazawa N, Yamamoto Y, Ohta C, Nishide T, Tsujioka K, Hattori M, Uede K, Hata M
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Journal Title
Clinical and Experimental Dermatology 36
Pages: 165-168
Peer Reviewed
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[Journal Article] Role of NOD2 genotype in the clinical phenotype of Blau syndrome and early-onset sarcoidosis.2009
Author(s)
Okafuji I, Nishikomori R, Kanazawa N, Kambe N, Fujisawa A, Yamazaki S, Saito M, Yoshioka T, Kawai T, Sakai H, Tanizaki H, Heike T, Miyachi Y, Nakahata T
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Journal Title
Arthritis and Rheumatism 60
Pages: 242-350
Peer Reviewed
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