2010 Fiscal Year Final Research Report
Optical Imaging of Intratumoral HIF-1 activity to Optimize Treatment Regimens for Chemoradiotherapy
| Project/Area Number |
21791184
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Radiation science
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| Research Institution | Kyoto University |
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Principal Investigator |
HARADA Hiroshi Kyoto University, 生命科学系キャリアパス形成ユニット, 講師 (80362531)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 放射線 / 腫瘍(がん) / 低酸素 / 微小環境 / 光イメージング |
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| Research Abstract |
Here, I and my colleagues performed a series of optical imaging experiments to visualize intratumoral HIF-1 activity, and optimized some combination of radiation with antitumor drugs, which could modulate HIF-1 activity, such as a VEGF neutralizing agent, bevacizumab, and a HIF-1 inhibitor, YC-1.
1. Optical imaging experiments showed that administration of a VEGF-neutralizing agent, bevacizumab, temporally induced vascular normalization and decreased hypoxic fraction in tumor xenografts 1 day after the treatment. When radiation treatment was given in this timing, the combined treatment significantly delayed tumor growth compared to radiation therapy alone.
2. We found that administration of a HIF-1 inhibitor, YC-1, suppressed radiation-induced activation of intratumoral HIF-1 activity, and then significantly delayed the growth of recurrent tumor after radiation therapy.
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[Journal Article] AHR, a novel acute hypoxia-response sequence, drives reporter gene under hypoxia in vitro and in vivo.2011
Author(s)
Cilek MZ, Hirohata S, Hatipoglu OF, Ogawa H, Miyoshi T, Inagaki J, Ohtsuki T, Harada H, Kamikawa S, Kusachi S, Ninomiya Y.
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Journal Title
Cell Biol Int. 35
Pages: 1-8
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[Journal Article] A novel strategy to tag matrix metalloproteinases-positive cells for in vivo imaging of invasive and metastatic activity of tumor cells.2010
Author(s)
Zhao T, Harada H, Teramura Y, Tanaka S, Itasaka S, Moriniibu A, Shinomiya K, Zhu Y, Hanaoka H, Iwata H, Saji H, Hiraoka M.
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Journal Title
J Control Release. 144
Pages: 109-114
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[Journal Article] Monitoring of biological one-electron reduction by 19F-NMR using hypoxia selective activation of a 19F-labeled indolequinone derivative.2009
Author(s)
Tanabe K, Harada H, Narazaki M, Tanaka K, Inafuku K, Komatsu H, Ito T, Yamada H, Chujo Y, Matsuda T, Hiraoka M, Nishimoto S.
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Journal Title
J Am Chem Soc. 131
Pages: 15982-15983
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[Journal Article] Angiogenic and vasoprotective effects of adrenomedulin on prevention of cognitive decline after chronic cerebral hypoperfusion in mice.
Author(s)
Maki T, Ihara M, Fujita Y, Nambu T, Miyashita K, Yamada M, Washida K, Nishio K, Ito H, Harada H, Yokoi H, Arai H, Itoh H, Nakao K, Takahashi R, Tomimoto H.
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