2010 Fiscal Year Final Research Report
Development of the high-sensitive imaging method for HIF-1-active hypoxic tumors based on the metabolic trapping approach
| Project/Area Number |
21791187
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Radiation science
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| Research Institution | Kyoto University |
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Principal Investigator |
UEDA Masashi Kyoto University, 医学研究科, 助教 (40381967)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 放射性医薬品 / 分子イメージング / 低酸素 / HIF-1 / 代謝捕捉 |
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| Research Abstract |
Hypoxia-inducible factor-1 (HIF-1) plays an important role in malignant tumor progression and in the development of resistance to radiotherapy. I designed a novel fusion protein (POTK) consisting of a protein transduction domain, herpes simplex virus type 1 thymidine kinase, and an essential part of the oxygen-dependent degradation domain of HIF-1 α that confers the same oxygen-dependent regulation as HIF-1α on POTK. I demonstrated that POTK and [^<123>I]FIAU, which is metabolized by POTK and trapped intracellularly, is potential probes for high-sensitive imaging of HIF-1-active regions in tumors.
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[Journal Article] Rapid detection of hypoxia-inducible factor-l-active tumours : pretargeted imaging with a protein degrading in a mechanism similar to hypoxia-inducible factor-lalpha.2010
Author(s)
Ueda M, Kudo T, Kuge Y, Mukai T, Tanaka S, Konishi H, Miyano A, Ono M, Kizaka-Kondoh S, Hiraoka M, Saji H.
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Journal Title
Eur J Nucl Med Mol Imaging. 37
Pages: 1566-74
Peer Reviewed
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[Journal Article] PET imaging of hypoxia-inducible factor-1-active tumor cells with pretargeted oxygen-dependent degradable streptavidin and a novel 18^F-labeled biotin derivative.
Author(s)
Kudo T, Ueda M, Konishi H, Kawashima H, Kuge Y, Mukai T, Miyano A, Tanaka S, Kizaka-Kondoh S, Hiraoka M, Sail H.
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Journal Title
Mol Imaging Biol. (in press)
Peer Reviewed
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