2010 Fiscal Year Final Research Report
Functional analysis of polycomb group gene Ring1B in the self-renewal of hepatic stem cells
| Project/Area Number |
21791258
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Yokohama City University |
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Principal Investigator |
UENO Yasuharu Yokohama City University, 医学部, 助教 (60375235)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 幹細胞 / 自己複製 / ポリコーム群タンパク質 / エピジェネティクス |
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| Research Abstract |
The polycomb-group (PcG) proteins are critical determinant for the self-renewal capacity of the stem cells by transcriptional repression of developmental regulatory genes. In this study, to investigate the role of PcG protein in maintaining the self-renewal capability function in the hepatic stem cells, we examined the Ring1B target gens in hepatic stem cells by using the Ring1B conditional KO mice and DNA microarrays. We demonstrated that Ring1B repressed approximately 900 genes in hepatic stem cell. Ring1B target gene sets contained multiple genes related to cell cycle and apoptosis. Ring1B may regulate the self-renewal capacity of hepatic stem cells by suppressing cell cycle genes and apoptosis regulatory genes.
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