2011 Fiscal Year Final Research Report

Approaches to regenerate pancreatic function by converting liver blood flow and transferring specific gene

Research Project

Project/Area Number	21791282
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Digestive surgery
Research Institution	Shiga University of Medical Science
Principal Investigator	FUJINO Kazunori 滋賀医科大学, 医学部, 助教 (70402716)
Project Period (FY)	2009 – 2011
Keywords	膵再生 / 遺伝子治療
Research Abstract	Although we found the both anti-insulin and anti-trypsin positive cells in the liver that interrupted portal blood flow by the immunohistochemical study, that immature cell cluster didn' t have any secretary granules. In this study, we attempted to introduce differentiation enhancing factor to the cluster by gene therapy, and generate pancreatic endocrine and exocrine cells. Although we try to make phage vector to deliver differentiation enhancing factor to the cluster for these research period, we couldn' t find adequate amount vector-transfected cells in both the liver and bile duct. We will continue to make vector for it in the future.