Identification of synovial sarcoma stem cell as a new therapeutic target

2010 Fiscal Year Final Research Report

• Project/Area Number: 21791383
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Orthopaedic surgery
• Research Institution: Hokkaido University
• Principal Investigator: KIMURA Taichi Hokkaido University, 大学院・医学研究科, 助教 (90435959)
• Project Period (FY): 2009 – 2010
• Keywords: 滑膜肉腫 / 癌幹細胞

Research Abstract

We attempted to identify a synovial sarcoma stem cell with sphere culture. The higher expression of stemness genes, Oct3, Nanog, and Sox2 was observed in the sphere subpopulation, and higher tumorigenicity was also observed in the xeno-plantation made from sphere subpopulation. Next, we compare the gene expression profiles between sphere and non-sphere subpopulation and determined candidate genes of the stem cell marker. Using the specific antibodies and cell sorter, we divided candidate gene positive and negative subpopulation, and analyzed the difference of tumorigenicity. We conformed that candidate gene positive subpopulation possess higher tumorigenicity than negative one.
In this study, we first detect synovial sarcoma stem cell and stem cell marker, and these findings play a very important role about exploitation the new therapy against synovial sarcoma.

Research Products

• [Journal Article] DOCK2 regulates cell proliferation through Rac and ERK activation in B cell lymphoma. (2010)
  • Author(s): Wang, L., Nishihara, H., Kato, Y., Kimura, T., Tanino, M., Nishio, M., Endo, T., Koike, T., Tanaka, S.
  • Journal Title: Biochem. Biophys. Res.Commun. 395 Pages: 111-115
  • Peer Reviewed
• [Journal Article] Analysis of an alternative human CD133 promoter reveals the implication of Ras/ERK pathway in tumor stem-like hallmarks. (2010)
  • Author(s): Tabu, K., Kimura, T., Sasai, K., Wang, L., Bizen, N., Nishihara, H., Taga, T., Tanaka, S.
  • Journal Title: Mol.Cancer. 9 Pages: 39-54
  • Peer Reviewed
• [Journal Article] Sudden death of a patient with pandemic influenza (A/H1N1pdm) virus infection by acute respiratory distress syndrome. (2010)
  • Author(s): Takiyama, A., Wang, L., Tanino, M., Kimura, T., Kawagishi, N., Kunieda, Y., Katano, H., Nakajima, N., Hasegawa, H., Takagi, T., Nishihara, H., Sata, T., Tanaka, S.
  • Journal Title: Jpn.J.Infect.Dis. 63 Pages: 72-74
  • Peer Reviewed
• [Journal Article] Colonic metastasis from primary carcinoma of the lung : report of a case and review of Japanese literature. (2009)
  • Author(s): Ono, H., Okabe, M., Kimura, T., Kawakami, M., Nakamura, K., Dnajo, Y., Takasugi, H., Nishihara, H.
  • Journal Title: Clin.J.Gastroenterol. 2 Pages: 89-95
  • Peer Reviewed
• [Journal Article] Human synovial sarcoma proto-oncogene Syt is essential for early embyonic development through the regulation of cell migration. (2009)
  • Author(s): Kimura, T., Sakai, M., Tabu, K., Wang, L., Tsunematsu, R., Tsuda, M., Sawa, H., Nagashima, K., Nishihara, H., Hatakeyama, S., Nakayama, K., Ladanyi, M., Tanaka, S., Nakayama, KI.
  • Journal Title: Lab.Invest. 89 Pages: 645-656
  • Peer Reviewed
• [Presentation] 腫瘍形成におけるoncogene-induced Senescence回避の解析 (2010)
  • Author(s): 高阪真路、笹井研、高橋健太、赤城剛、谷野美智枝、木村太一、西原広史、田中伸哉
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 20100922-20100924
• [Presentation] CrkL plays a pivotal role in tumoregenesis of the oral squamous cell carcinoma. (2010)
  • Author(s): Hiroko, Nakamura, Hiroshi, Nishihara, Taichi, Kimura, Kei, Wang, Satoshi, Fukuda, Shinya Tanaka.
  • Organizer: 第69回日本癌化学会学術総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 20100922-20100924
• [Presentation] DOCK2 regulates cell proliferation Through Rac and ERKactivation in B cell Lymphoma. (2010)
  • Author(s): Wang L,Nishihara H, kimura T, Tanino M,Nishio M, Obara M,Endo T, KoikeT, Tanaka S.
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 20100922-20100924
• [Presentation] Analysis of escape from oncogene-induced senescence in tumorigenesis (2010)
  • Author(s): Shinji Kohsaka, ken Sasai, Kenta Takahashi, Tsuyoshi Akagi, Mishie Tanino, Taichi Kimura, Hiroshi Nishihara, Shinya Tanaka.
  • Organizer: Cold Spring Harbor Laboratori Meeting ; Mechanisms and Models of Cancer
  • Place of Presentation: Cold Spring Harbor, USA
  • Year and Date: 20100817-20100821
• [Presentation] Analysis of stem-like cell features of human synovial sarcoma. (2010)
  • Author(s): Taichi kimura, Lei Wang, Miho Nodagashira, Hiroko Nakamura, Mishie Tanino, Hiroshi Nishihara, Shinya Tanaka.
  • Organizer: Cold Spring Harbor Laboratori Meeting ; Mechanisms and Models of Cancer
  • Place of Presentation: Cold Spring Harbor, USA
  • Year and Date: 20100817-20100821
• [Presentation] 捺印標本を用いたグリオーマのMGMT免疫染色の検討 (2010)
  • Author(s): 青柳瑛子、王磊、笹井研、谷野美智枝、木村太一、西原広史、藤本真、石井伸明、伊東民雄、田中伸哉
  • Organizer: 第51回日本臨床細胞学会総会(春期大会)
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 20100529-20100531
• [Presentation] 新WHO分類に基づmalignant glioma 65症例のreview:組織像と全生存率の関連について (2010)
  • Author(s): 菅野宏美、西原広史、谷野美智枝、木村太一、高橋健太、山口秀、成田拓人、小林浩之、寺坂俊介、田中伸哉
  • Organizer: 第28回日本脳腫瘍病理学会
  • Place of Presentation: 大阪市中央公会堂
  • Year and Date: 20100521-20100522
• [Presentation] 神経症状を初発とし診断に苦慮した血管内リンパ腫の一例 (2010)
  • Author(s): 泉真祐子、長谷川祐太、高阪真路、谷野美智枝、木村太一、古山裕康、千葉進、及川光照、西原広史、田中伸哉
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 20100427-20100429
• [Presentation] 放射線治療後7年間生存し得た原発性悪性心膜中皮腫の一例 (2010)
  • Author(s): 谷野美智枝、ロシャン・マハビール、菅野宏美、鈴木宏明、山城勝重、木村太一、西原広史、丸川活史、松野吉宏、田中伸哉
  • Organizer: 第99回日本病理学会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 20100427-20100429
• [Presentation] 神経膠腫のパラフィン包埋切片を用いたFISH法による1番短腕の欠失の遺伝子解析と予後の検討 (2010)
  • Author(s): 佐藤真実、谷野美智枝、木村太一、西原広史、伊東民雄、佐和広基、金子貞男、村田純一、加藤正仁、田中伸哉
  • Organizer: 第99回日本病理学会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 20100427-20100429
• [Presentation] Gliomaに特徴的な血管構造の臨床病理学的解析 (2010)
  • Author(s): 菅野宏美、西原広史、谷野美智枝、木村太一、田中伸哉
  • Organizer: 第99回日本病理学会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 20100427-20100429
• [Presentation] 脊髄腫瘍として発見され、ユーイング肉腫との鑑別を要したmyeloid sarcomaの一例 (2010)
  • Author(s): 湯澤明夏、柴田ひな、菅野宏美、谷野美智枝、矢野俊介、木村太一、西原広史、田中伸哉
  • Organizer: 第99回日本病理学会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 20100427-20100429
• [Presentation] 新型インフルエンザ(A/H1N1pdm)肺炎によるびまん性肺胞傷害により急死した1剖検例 (2010)
  • Author(s): 瀧山晃弘、王磊、谷野美智枝、木村太一、西原広史、川岸直樹、國枝保幸、片野晴隆、長谷川秀樹、高木知敬、佐多徹太郎、田中伸哉
  • Organizer: 第99回日本病理学会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 20100427-20100429
• [Presentation] シグナル伝達分子のImmunoprofiling;胃癌20例における臨床病理学的検討 (2010)
  • Author(s): 石川麻倫、大場彩音、西原広史、菅野宏美、木村太一、谷野美智枝、田中伸哉
  • Organizer: 第99回日本病理学会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 20100427-20100429
• [Presentation] Quantitative Proteome Analysis in Transformed Astrocyte Cell using Spectral Counting and SILAC Methods. (2009)
  • Author(s): Kiyonaga Fujii, Ken Sasai, Taichi Kimura, Shinya Tanaka, Fuyuhiko Inagaki.
  • Organizer: Pennsylvanvia Convention Center.
  • Place of Presentation: Philadelphia, Pennsylvania, USA
  • Year and Date: 20090531-20090604
• [Presentation] 滑膜肉腫細胞株におけるCD133の機能解析 (2009)
  • Author(s): 木村太一、王磊、平賀博明、西原広史、田中伸哉
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 20090501-20090503
• [Presentation] 間質性肺炎の経過中、急性に呼吸不全が悪化し死亡された11剖検例の検討 (2009)
  • Author(s): 谷野美智枝、高阪真路、木村太一、西原広史、進藤正信、田中伸哉
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 20090501-20090503
• [Presentation] Clostridium属によるガス壊疽により死亡した2剖検例 (2009)
  • Author(s): 石川麻倫、柴田頌太、谷野美智枝、木村太一、西原広史、篠原敏也、田中伸哉
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 20090501-20090503
• [Presentation] hCG産生大腸癌の分子病理学的検討 (2009)
  • Author(s): 吉永智彰、西原広史、福島祐介、佐和弘基、村上普美、木村太一、谷野美智枝、田中伸哉
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 20090501-20090503
• [Presentation] グリオーマにおかえるOlig2発現制御機構と機能解析 (2009)
  • Author(s): 野田頭未歩、笹井研、木村太一、青柳瑛子、田中伸哉
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 20090501-20090503