2010 Fiscal Year Final Research Report
Impact of anesthetic-induced preconditioning on mitochondrial ion channels
| Project/Area Number |
21791427
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
TAMPO Akihito Asahikawa Medical College, 医学部, 助教 (80531524)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 蘇生学 |
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| Research Abstract |
We studied electrophysiological profiles of mitochondrial ion channels, which are known as important factors in anesthetic-induced preconditioning. Mytoplasts were obtained from isolated rat hearts by osmotic shock. Mitochondrial KATP channel were not detected in this study. We identified a mitochondrial ion channel with a conductance of 110pS in equimolar 150mM KCL solution. This channel current was removed when the 150mM KCL bath solution was replaced with 150mM K-glutamate. When 150mM TEA-Cl was used as bath solution, the current was not affected. In addition, The current was inhibited by DIDS, a chloride channel blocker. These results suggested that the mitochondrial ion channel was IMAC (inner membrane anion channel).
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