2010 Fiscal Year Final Research Report
Development of new gene knockdown techniques for platelets using miRNA
| Project/Area Number |
21791466
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
KATO Yuko Kyoto Prefectural University of Medicine, 医学部附属病院, 専攻医 (50398400)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 周術期管理学 / 遺伝子治療 |
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| Research Abstract |
After transfection of miR RNAi expression vector in human CD34+ progenitor cells and mouse bone marrow cells along with differentiating into platelets with 3-4week culture in vitro, we have confirmed the target gene knocked down in platelet cells. These platelets have less ability of aggregation and P-selectin expression in response to agonists. With injection of these gene knocked down platelets, survival and severity of pulmonary embolism have improved in mouse experimental model. Further studies are necessary to undergo detailed analyses.
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