Identification of perineural invasion inducing factors in prostate cance

2012 Fiscal Year Final Research Report

• Project/Area Number: 21791483
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Urology
• Research Institution: Hirosaki University
• Principal Investigator: OKAMOTO Akiko 弘前大学, 医学(系)研究科(研究院), 助教 (60436037)
• Project Period (FY): 2009 – 2012
• Keywords: 前立腺癌 / 神経浸潤 / 分子機構

Research Abstract

Perineural invasion (PNI) has been demonstrated to be one of the major mechanisms of extension of prostate cancer from the prostatic parenchyma to the periprostatic soft tissue. Patients with PNI have higher risk of recurrent disease. However, the mechanisms of PNI are not well known. Here, we investigated PNI inducing factors and surface molecules on human neuron cells. Results : Motility and invasion activity of DU145 (Prostate cancer cell line) were increased when co-cultured with neuroendocrine cell line PC12 (pheochromocytoma of the rat adrenal medulla). SDF-1 was identified as cytokine from human primary neuron (HN) cells. Because prostate cancer expresses CXCR4, and CXCR4 is reported as a receptor of SDF-1, SDF-1 in neuron and CXCR4 in prostate cancer has potential to induce PNI in prostate cancer. Surface protein screening of HN cells using the DU145 cDNA phage display library, which allows displaying recombinant DU145 tumor proteins on the surface of T7 phage, showed integrin a6 as a candidate of PNI inducing molecule. Further analyses are necessary to identify the PNI inducing factors in prostate cancer.

Research Products

• [Journal Article] Effect of an Oral Adsorbent, AST-120, on Dialysis Initiation and Survival in Patients with Chronic Kidney Disease (2012)
  • Author(s): Hatakeyama S, Yamamoto H, Okamoto A, Imanishi K, Tokui N, Okamoto T, Suzuki Y, Sugiyama N, Imai A, Kudo S, Yoneyama T, Hashimoto Y, Koie T, Kamimura N, Saitoh H, Funyu T, hyama C
  • Journal Title: Int. J Nephrol Pages: 1-7
  • DOI: doi:10.1155/2012/385274
  • Peer Reviewed
• [Journal Article] Biochemical outcome of small-volume or insignificant prostatecancer treated with radical prostatectomy in Japanese population (2011)
  • Author(s): Hashimoto Y, Okamoto A, Imai A, Yoneyama T, Hatakeyama S, Yoneyama T, Koie T, Kaminura N, Ohyama C
  • Journal Title: Int. J. Clin. Oncol Volume: 17 Pages: 119-127
  • DOI: doi:10.1007/s10147-011-0267-6
  • Peer Reviewed
• [Journal Article] An aggressive signet ring cell carcinoma of the prostate in a Japanese man (2011)
  • Author(s): Hashimoto Y, Imanishi K, Okamoto A, Sasaki A, Saitoh H, Wada R, Yamamoto H, Koie T, Ohyama C
  • Journal Title: Case Rep Oncol Volume: 4 Pages: 517-520
  • DOI: doi:10.1159/000334081
  • Peer Reviewed
• [Journal Article] Efficacy of SMART Stent Placement for Salvage Angioplasty in Hemodialysis Patients with Recurrent Vascular Access Stenosis (2011)
  • Author(s): Hatakeyama S, Toikawa T, Okamoto A, Yamamoto H, Imanishi K, Okamoto T, Tokui N, Suzuki Y, Sugiyama N, Imai A, Hashimoto Y, Kudo S, Yoneyama T, Koie T, Kamimura N, Saitoh H, Funyu T, Ohyama C
  • Journal Title: Int J Nephrol Pages: 1-6
  • DOI: doi:10.4061/2011/464735
  • Peer Reviewed
• [Journal Article] Carboplatin-gemcitabine combination chemotherapy upregulates AKR1B10 expression in bladder cancer (2011)
  • Author(s): Hashimoto Y, Imanishi K, Tokui N, Okamoto T, Okamoto A, Hatakeyama S, Yoneyama T, Koie T, Kamimura N, Ohyama C
  • Journal Title: Int J Clin Oncol Volume: 18 Pages: 177-182
  • DOI: doi:10.1007/s10147-011-0363-7
  • Peer Reviewed
• [Journal Article] Core 2 N-acetylglucosaminyltransferase-1 (C2GnT-1) expression induces aggressive potential of testicular germ cell tumor (2010)
  • Author(s): Hatakeyama S, Kyan A, Yamamoto H, Okamoto A, Sugiyama N, Suzuki Y, Yoneyama T, Hashimoto Y, Koie T, Yamada S, Saito H, Arai Y, Fukuda M, Ohyama C
  • Journal Title: Int J Cancer Volume: 127 Pages: 1052-1059
  • DOI: doi:10.1002/ijc.25117
  • Peer Reviewed
• [Journal Article] Invadopodia formation by bladder tumor cells (2010)
  • Author(s): Sutoh M, Hashimoto Y, Yoneyama T, Yamamoto H, Hatakeyama S, Koie T, Okamoto A, Yamaya K, Saitoh H, Funyu T, Nakamura T, Sato T, Ohyama C, Tsuboi S
  • Journal Title: Oncology Research Volume: 19 Pages: 85-92
  • Peer Reviewed
• [Journal Article] 前立腺全摘除術標本における腫瘍体積とPSA再発との関連について (2010)
  • Author(s): 橋本安弘,百瀬昭志,岡本亜希子,山本勇人,畠山真吾,岩渕郁哉,米山高弘,古家琢也,神村典孝,大山 力
  • Journal Title: 泌尿器科紀要 Volume: 56 Pages: 91-94
  • Peer Reviewed
• [Journal Article] Protein profiling of post-prostatic massage urine specimens by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry to discriminate between prostate cancer and benign lesions (2009)
  • Author(s): Okamoto A, Yamamoto H, Imai A, Hatakeyama S, Iwabuchi I, Yoneyama T, Hashimoto Y, Koie T, Kamimura N, Mori K, Yamaya K, Ohyama C
  • Journal Title: ONCOLOGY REPORTS Volume: 21 Pages: 73-79
  • Peer Reviewed
• [Presentation] Detection of prostate cancer associated α2,3 sialyated free PSA in serum is more higher specificity than conventional PSA test (2013)
  • Author(s): Yoneyama To, Tobisawa Y, Mori K, Hatakeyama S, Yamamoto H, Okamoto T, Okamoto A, Ishimura H, Yoneyama Ta, Koie T, Kamimura N,Ohyama C
  • Organizer: EAU(European Association of Urology)
  • Place of Presentation: ミラノ、イタリア
  • Year and Date: 20130315-19
• [Presentation] Anti-hypertensive effect of laparoscopic adrenalectomy (LA) for the patients with primary aldosteronism (PA) is limited despite of the accurate determinationof responsible lesion by adrenal venous sampling (AVS) (2013)
  • Author(s): Yamamoto H, Hatakeyama S, Sugiyama N, Suzuki Y, Okamoto A, Imai A, Yoneyama Ta, Hashimoto Y, Koie T, Nigawara T, Terui K, Sakihara S, OhyamaC
  • Organizer: EAU(European Association of Urology)
  • Place of Presentation: ミラノ、イタリア
  • Year and Date: 20130315-19
• [Presentation] Sequential chemotherapy with Gemcitabine plus Carboplatin followed by additional Docetaxel for aged patients with advanced upper-tract urotherial cancer (2013)
  • Author(s): Yoneyama Ta, Hagiwara K, Oikawa M, Suzuki Y, Sugiyama N, Tobisawa Y, Yoneyama To, Okamoto A, Yamamoto H, Imai A, Hatakeyama S, Mori K, Hashimoto Y, Koie T, Ohyama C
  • Organizer: EAU(European Association of Urology)
  • Place of Presentation: ミラノ、アタリア
  • Year and Date: 20130315-19
• [Presentation] Sequential chemotherapy with Gemcitabine+Carboplatin followed by additional Docetaxel for aged patients with advanced bladder cancer (2013)
  • Author(s): Yoneyama Ta, Hagiwara K, Oikawa M, Suzuki Y, Sugiyama N, Tobisawa Y, Yoneyama To, Okamoto A, Yamamoto H, Imai A, Hatakeyama S, Mori K, Hashimoto Y, Koie T, Ohyama C
  • Organizer: EAU(European Association of Urology)
  • Place of Presentation: ミラノ、イタリア
  • Year and Date: 20130315-19
• [Presentation] Neoadjuvant gemcitabine plus carboplatin followed by immediate with muscleinvasive bladder cancer : A propensity score analysis (2012)
  • Author(s): Koie T, Okamoto A, Yamamoto H, Tobisawa Y, Yoneyama T, Mori K, Hatakeyama S, Yoneyama T, Hashimoto Y, Kamimura N, Ohyama C
  • Organizer: 第107回米国泌尿器科学会(AUA)
  • Place of Presentation: アトランタ,USA
  • Year and Date: 20121913
• [Presentation] Detection of prostate cancer-associated N-glycan on free PSA by Luminex bead-based immunoassay : A novel prostate cancer screening method with higher specificity than conventional PSA test (2012)
  • Author(s): Yoneyama Tohru, Tobisawa Y, Mori K, Hatakeyama S, Yamamoto H, Okamoto A, Ishimura F, Yoneyama Takahiro, Koie T, Kamimura N, Ohyama C
  • Organizer: Glycobiology Meeting
  • Place of Presentation: サンディエゴ、アメリカ
  • Year and Date: 20121111-14
• [Presentation] ハイリスク前立腺癌に対するLHRH+エストラムスチンの有用性-プロペンシティスコア解析 (2012)
  • Author(s): 古家琢也,三塚浩二,成田伸太郎,米山高弘,川村貞文,岡本亜希子,山本勇人,今井 篤,畠山真吾,橋本安弘,栃木達夫,羽渕友則,荒井陽一,大山 力
  • Organizer: 第50回日本癌治療学会学術集会
  • Place of Presentation: 横浜市(パシフィコ横浜)
  • Year and Date: 20121025-27
• [Presentation] 前立腺癌の遊走能と湿潤脳における神経細胞の役割について (2012)
  • Author(s): 岡本亜希子,畠山真吾,米山美穂子,米山 徹,村澤洋美,岩村大径,飛澤悠葵,山本勇人,米山高弘,橋本安弘,古家琢也,神村典孝,大山 力
  • Organizer: 第77回日本泌尿器科学会東部総会平成
  • Place of Presentation: 文京区(東京ドームホテル)
  • Year and Date: 20121017-19
• [Presentation] 高リスク前立腺癌に対するネオアジュバント内分泌化学療法後のロボット支援前立腺全摘除術の経験 (2012)
  • Author(s): 米山高弘,佐藤天童,三上穣太郎,福士謙,飛澤悠葵,米山 徹,岡本亜希子,山本勇人,今井 篤,畠山真吾,古家琢也,神村典孝,大山 力
  • Organizer: 第77回日本泌尿器科学会東部総会
  • Place of Presentation: 文京区(東京ドームホテル)
  • Year and Date: 20121017-19
• [Presentation] 前立腺癌に対する小線源療法後の下部尿路症状に対するα1ブロッカーの効果 (2012)
  • Author(s): 今井 篤,藤田尚紀,福士 謙,三上穣太郎,佐藤天童,村澤洋美,山本勇人,岡本亜希子,畠山真吾,米山高弘,古家琢也,神村典孝,大山 力
  • Organizer: 第77回日本泌尿器科学会東部総会
  • Place of Presentation: 文京区(東京ドームホテル)
  • Year and Date: 20121017-19
• [Presentation] 前立腺全摘除術後の下部尿路症状の経時的変化に関する検討 (2012)
  • Author(s): 山本勇人,岩村大径,小島由太,佐藤天童,村澤洋美,岡本亜希子,今井 篤,畠山真吾,米山高弘,古家琢也,神村典孝,大山 力
  • Organizer: 第77回日本泌尿器科学会東部総会
  • Place of Presentation: 文京区(東京ドームホテル)
  • Year and Date: 20121017-19
• [Presentation] Safty and Efficacy of Neoadjuvant Gemcitabine plus Carboplatin Followed by Immediate Cystectomy in Patients with Muscle-Invasive Bladder Cancer (2012)
  • Author(s): Koie T, Okamoto A, Yamamoto H, Tobisawa Y, Yoneyama Tohru, Mori K, Hatakeyama S, Yoneyama Takahiro, Hashimoto Y, Kamimura N, Ohyama C
  • Organizer: 第32回国際泌尿器科学会総会(SIU)
  • Place of Presentation: 福岡市(福岡国際会議場)
  • Year and Date: 20120930-1004
• [Presentation] Type of androgen deprivation therapy affects metabolic condition and adipose tissue distribution (2012)
  • Author(s): Hatakeyama S, Yamamoto H, Murasawa H, Okamoto A, Imai A, Yoneyama Takahiro, Hashimoto Y, Koie T, Kamimura N, Ohyama C
  • Organizer: 第32回国際泌尿器科学会総会(SIU)
  • Place of Presentation: 福岡市(福岡国際会議場)
  • Year and Date: 20120930-1004
• [Presentation] RALPと同じポートポジションでのロボット支援腹腔鏡下膀胱全摘除術(RALC) (2012)
  • Author(s): 畠山真吾,岡本亜希子,山本勇人,今井篤,米山高広,古家琢也,神村典孝,大山 力
  • Organizer: 第26回日本泌尿器内視鏡学会総会
  • Place of Presentation: 仙台市(仙台国際センター)
  • Year and Date: 2012-11-24
• [Presentation] ロボット支援腹腔鏡下根治的前立腺全摘除術初期20例の成績 (2012)
  • Author(s): 古家琢也,岡本亜希子,山本勇人,畠山真吾,米山高広,橋本安弘,大山 力
  • Organizer: 第26回日本泌尿器内視鏡学会総会
  • Place of Presentation: 仙台市(仙台国際センター)
  • Year and Date: 2012-11-24
• [Presentation] 当科におけるロボット前立腺全摘除術の成績 (2012)
  • Author(s): 古家琢也,萩原和久,成田琢磨,及川真亮,鈴木裕一朗,杉山尚樹,米山 徹,飛澤悠葵,盛 和行,岡本亜希子,山本勇人,今井 篤,畠山真吾,米山高弘,橋本安弘,大山 力
  • Organizer: 第37回青森県泌尿器科研究会
  • Place of Presentation: 青森市(青森グランドホテル)
  • Year and Date: 2012-11-10
• [Presentation] 前立腺癌骨転移との鑑別に難渋した骨パジェット病の1例 (2012)
  • Author(s): 福士 謙,岩村大径,小島由太,佐藤天童,山本勇人,岡本亜希子,今井 篤,畠山真吾,石村大史,米山高弘,古家琢也,神村孝典,大山 力
  • Organizer: 第246回日本泌尿器科学会東北地方会
  • Place of Presentation: 福島市(コラッセふくしま)
  • Year and Date: 2012-09-29
• [Presentation] Core3 O-Glycan Synthase Suppresses Tumor Formation and Metastasis of Prostate Carcinoma PC3 cells through Down-regulation of Integrin Complex (2010)
  • Author(s): Hatakeyama S., Lee SH., Yamamoto H, Okamoto A, Okamoto T, Imanishi K, Suzuki Y, Sugiyama N, Kudo S, Yoneyama T, Hashimto Y, Koie T, Kamimura N, Ohyama C, Fukuda M
  • Organizer: 25th Annual EAU Congress Barcelona
  • Place of Presentation: Spain
  • Year and Date: 20100416-20
• [Presentation] Core 2 N-acetylglucosaminyltransferase-1 (C2GnT-1) expression induces aggressive potential of testicular germ cell tumor (2010)
  • Author(s): Hatakeyama S, Kyan A, Yamamoto H, Okamoto.A, Sugiyama N, Suzuki Y, Imanishi K, Okamoto T, Kudo S, Yoneyama T, Hashimto Y, Koie T, Kamimura N, Ohyama C
  • Organizer: 25th Annual EAU Congress Barcelona
  • Place of Presentation: Spain
  • Year and Date: 20100406-20