2011 Fiscal Year Annual Research Report
尿路結石形成防御における腎マクロファージの機能について
Project/Area Number |
21791517
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Research Institution | Nagoya City University |
Principal Investigator |
岡田 淳志 名古屋市立大学, 大学院・医学研究科, 助教 (70444966)
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Keywords | 尿路結石 / マクロファージ / op/op / オステオポンチン / グリオキシル酸 / M-CSF |
Research Abstract |
1.マクロファージ機能不全マウス(op/op)に対するM-CSFの結石予防効果 昨年度までの研究で、op/opマウスに対してグリオキシル酸投与を実施した結果、op/opの腎結石はwild-typeと比較して有意に多く形成され、さらに結石は乳頭部に集簇するというこれまでの実験モデルに見られない現象を認めた。 本年度は、このモデルに対し、op/opにおけるマクロファージ機能不全の原因となっているcolony stimulating factor 1遺伝子変異の性質を補完するために、M-CSF(macrophage colony stimulating factor)を計非投与することによって結石形成が減少するかどうかを検証した。その結果、M-CSFは濃度依存的にop/opマウスで増加した結石を抑制することが判明した。 2.培養マクロファージを用いた腎結晶貧食研究 前年度では、マウス培養腎尿細管細胞(M-1)およびマウス培養マクロファージ細胞(J774.1)を用いた単離培養における各細胞のシュウ酸カルシウム-水和物(COM)結晶の貧食能についての観察と定量法の確立を行い、M-1と比較し、J774.1の結晶貧食能が有意に高いことを示した。 本年度は、貧食された結晶が細胞内のどの器官に取り込まれているかを証明するため、Lysotracker試薬を用いて、貧食結晶がJ774.1のみリソゾームに取り込まれている可能性を示した。また細胞分裂の過程で、COM結晶も同様に分配・消失していく像を捉えた。
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Research Products
(55 results)
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[Journal Article] Mitocondrial permeability transition pore opening induces the initial process of renal calcium crystallization2012
Author(s)
Niimi Kazuhiro, Yasui Takahiro, Hirose Masahito, Hamamoto Shuzo, Itoh Yasunori, Okada Atsushi, Kubota Yasue, Kojima Yoshiyuki, Tozawa Keiichi, Sasaki Shoichi, Hayashi Yutaro, Kohri Kenjiro
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Journal Title
Free Radical Biology & Medicine
Volume: 52
Pages: 1207-1217
DOI
Peer Reviewed
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[Journal Article] The role of long-term loading of cholesterol in renal crystal formation2011
Author(s)
Itoh Yasunori, Yoshimura Mugi, Niimi Kazuhiro, Usami Masayuki, Hamamoto Shuzo, Kobayashi Takahiro, Hirose Masahito, Okada Atsushi, Yasui Takahiro, Tozawa Keiichi, Kohri Kenjiro
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Journal Title
Archivio Italiano di Urologia e Andrologia
Volume: 83
Pages: 23-25
Peer Reviewed
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[Journal Article] Crucial role of the cryptic peitope SLAYGLR within osteopontin in renal crystal formation of mice2011
Author(s)
Hamamoto Shuzo, Yasui Takahiro, Okada Atsushi, Hirose Masahito Matsui Yutaka, Kon Shigeyuki, Sakai Fumihiko, Kojima Yoshiyuki, Hayashi Yutaro, Tozawa Keiichi, Ueda Toshimitsu, Kohri Kenjiro
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Journal Title
Journal of Bone and Mineral Research
Volume: 26
Pages: 2967-2977
DOI
Peer Reviewed
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[Presentation] Adiponectin is a possible inhibitor for metabolic syndrome induced kidney stone formation using ob/ob mouse2012
Author(s)
Okada Atsushi, Taguchi Kazumi, Hirose Yasuhiko, Niimi Kazuhiro, Fujii Yasuhiro, Kobayashi Takahiro, Usami Masayuki, Hamamoto Shuzo, Hirose Masahito, Itoh Yasunori, Yasui Takahiro, Tozawa Keiichi, Kohri Kenjiro
Organizer
European Association of Urology Annual Congress 2012
Place of Presentation
Le Palais des Congres de Paris(フランス)
Year and Date
20120224-20120228
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[Presentation] M-CSF is a novel preventive agent against renal stone disease by inducing anti-inflammatory macrophages2012
Author(s)
Taguchi Kazumi, Okada Atsushi, Fujii Yasuhiro, Niimi Kazumi, Kobayashi Takahiro, Hamamoto Shuzo, Hirose Masahito, Itoh Yasunori, Yasui Takahiro, Tozawa Keiichi, Sasaki Shoichi, Hayashi Yutaro, Kohri Kenjiro
Organizer
European Association of Urology Annual Congress 2012
Place of Presentation
Le Palais des Congres de Paris(フランス)
Year and Date
20120224-20120228
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[Presentation] Efficacy of cornier official technical training for SWL treatment at the university hospital2012
Author(s)
Hamamoto Shuzo, Okada Atsushi, Taguchi Kazumi, Hirose Yasuhiko, Niimi Kazumi, Fujii Yasuhiro, Ando Ryosuke, Kobayashi Takahiro, Usami Masayuki, Hirose Masahito, Itoh Yasunori, Yasui Takahiro, Tozawa Keiichi, Kohri Kenjiro
Organizer
European Association of Urology Annual Congress 2012
Place of Presentation
Le Palais des Congres de Paris(フランス)
Year and Date
20120224-20120228
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[Presentation] Opening of mitochondrial permeability transition pores induces kidney stone formation2011
Author(s)
Niimi Kazuhiro, Yasui Takahiro, Taguchi Kazumi, Fujii Yasuhiro, Hirose Yasuhiko, Kobayashi Takahiro, Hamamoto Shuzo, Hirose Masahito, Okada Atsushi, Itoh Yasunori, Tozawa Keiichi, Kohri Kenjiro
Organizer
1st Meeting of the EAU Section of Urolithiasis (EULIS)
Place of Presentation
The Mermaid Conference & Events Center(イギリス)
Year and Date
20110907-20110910
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[Presentation] Efficacy of a selective alpha 1A adrenoceptor antagonist as a medical exclusive therapy against ureteral stones2011
Author(s)
Ito Yasunori, Okada Atsushi, Yasui Takahiro, Taguchi Kazumi, Niimi Kazuhiro, Fujii Yasuhiro, Hirose Yasuhiko, Usami Masayuki, Hamamoto Shuzo, Hirose Masahito, Fujita Keiji, Tozawa Keiichi, Kohri Kenjiro
Organizer
1st Meeting of the EAU Section of Urolithiasis (EULIS)
Place of Presentation
The Mermaid Conference & Events Center(イギリス)
Year and Date
20110907-20110910
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[Presentation] Bisphosphonate prevents urolithiasis in men with osteoporosis2011
Author(s)
Yasui Takahiro, Okada Atsushi, Ichikawa Jun, Taguchi Kazumi, Hirose Yasuhiko, Fujii Yasuhiro, Niimi Kazuhiro, Usami Masayuki, Ando Ryosuke, Hamamoto Shuzo, Kobayashi Takahiro, Hirose Masahito, Itoh Yasunori, Tozawa Keiichi, Kohri Kenjiro
Organizer
1st Meeting of the EAU Section of Urolithiasis (EULIS)
Place of Presentation
The Mermaid Conference & Events Center(イギリス)
Year and Date
20110907-20110910
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[Presentation] Calcium oxalate crystals could be englobed by macrophages during kidney stone formation in vitro and in vivo models2011
Author(s)
Fujii Yasuhiro, Okada Atsushi, Ichikawa Jun, Niimi Kazuhiro, Kobayashi Takahiro, Hamamoto Shuzo, Hirose Masahito, Itoh Yasunori, Yasui Takahiro, Tozawa Keiichi, Kohri Kenjiro
Organizer
1st Meeting of the EAU Section of Urolithiasis (EULIS)
Place of Presentation
The Mermaid Conference & Events Center(イギリス)
Year and Date
20110907-20110910
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[Presentation] Renal macrophage migration and crystal phagocytosis via inflammatory-related gene expression during kidney stone formation and elimination in mice2011
Author(s)
Okada Atsushi, Yasui Takahiro, Ichikawa Jun, Nakaoka Kazunori, Hirose Yasuhiko, Taguchi Kazumi, Niimi Kazuhiro, Fujii Yasuhiro, Usami Masayuki, Kobayashi Takahiro, Ando Ryosuke, Hamamoto Shuzo, Hirose Masahito, Itoh Yasunori, Tozawa Keiichi, Kohri Kenjiro
Organizer
American Urological Association Annual Meeting 2011
Place of Presentation
Washington Convention Center (U.S.A)
Year and Date
20110514-20110519
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[Presentation] Pioglitazone, a peroxisome proliferator-activated receptor gamma γ (PPAR-γ) activator, possibly inhibits renal stone formation in rats2011
Author(s)
Taguchi Kazumi, Okada Atsushi, Fujii Yasuhiro, Niimi Kazuhiro, Kobayashi Takahiro, Hamamoto Shuzo, Hirose Masahito, Itoh Yasunori, Yasui Takahiro, Tozawa Keiichi, Sasaki Shoichi, Hayashi Yutaro, Kohri Kenjiro
Organizer
American Urological Association Annual Meeting 2011
Place of Presentation
Washington Convention Center (U.S.A)
Year and Date
20110514-20110519
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[Presentation] Association between urolithiasis and food intake based on data from a Japanese national survey2011
Author(s)
Yasui Takahiro, Okada Atsushi, Taguchi Kazumi, Hirose Yasuhiko, Fujii Yasuhiro, Niimi Kazuhiro, Usami Masayuki, Ando Ryosuke, Hamamoto Shuzo, Kobayashi Takahiro, Hirose Masahito, Itoh Yasunori, Tozawa Keiichi, Kohn Kenjiro
Organizer
American Urological Association Annual Meeting 2011
Place of Presentation
Washington Convention Center (U.S.A)
Year and Date
20110514-20110519
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[Presentation] Macrophage-colony stimulating factor deficient mice demonstrate that macrophages play a defensive role against renal stone formation2011
Author(s)
Taguchi Kazumi, Okada Atsushi, Itoh Yasuhiro, Niimi Kazuhiro, Kobayashi Takahiro, Hamamoto Syuzo, Hirose Masahito, Itoh Yasunori, Yasui Takahiro, Tozawa Keiichi, Sasaki Shoichi, Hayashi Yutaro, Kohri Kenjiro
Organizer
American Urological Association Annual Meeting 2011
Place of Presentation
Washington Convention Center (U.S.A)
Year and Date
20110514-20110519
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[Presentation] Bisphosphonate prevents the recurrence of urolithiasis in postmenopausal woman with osteoporosis2011
Author(s)
Yasui Takahiro, Okada Atsushi, Taguchi Kazumi, Hirose Yasuhiko, Fujii Yasuhiro, Niimi Kazuhiro, Usami Masayuki, Ando Ryosuke, Hamamoto Shuzo, Kobayashi Takahiro, Hirose Masahito, Itoh Yasunori, Tozawa Keiichi, Kohri Kenjiro
Organizer
American Urological Association Annual Meeting 2011
Place of Presentation
Washington Convention Center (U.S.A)
Year and Date
20110514-20110519
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