2011 Fiscal Year Final Research Report
The role of macrophages for prevention of kidney stone formation
| Project/Area Number |
21791517
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Urology
|
|---|
| Research Institution | Nagoya City University |
|---|
Principal Investigator |
OKADA Atsushi 名古屋市立大学, 大学院・医学研究科, 助教 (70444966)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Keywords | 尿路結石 / マクロファージ / op/op / M-CSF |
|---|
| Research Abstract |
The present study was performed for investigation of kidney stone prevention effect using M-CSF deficient mice(op/op) and observation of COM crystals phagocytosis using cultured macrophages.
(1) Using op/op mice in which macrophage differentiation and migration cannot occurred by macrophage-colony stimulating factor(M-CSF) gene deficient, increased stone formation were detected. And exogenous M-CSF administration old lead decrease of stone formation.
(2) cells(M-1), COM crystal exposure was performed in vitro. As the results, COM phagocytosis rate of J774.1 were significantly higher than that of M-1.And englobed crystals were detected in the lysosomes in macrophages. Moreover, the crystals were getting reduced during the experiment.
Taken together, the possible function of macrophages for inhibition of kidney stone formation by phagocytosis and processing renal crystals.
|
Research Products
(43 results)
-
[Journal Article] Mitochondrial permeability transition pore opening induces the initial process of renal calcium crystallization2012
Author(s)
Niimi K, Yasui T, Hirose M, Hamamoto S, Itoh Y, Okada A, Kubota Y, Kojima Y, Tozawa K, Sasaki S, Hayashi Y, Kohri K
-
Journal Title
Free Radical Biology & Medicine
Volume: 52
Pages: 1207-1217
Peer Reviewed
-
-
-
[Journal Article] The role of long-term loading of cholesterol in renal crystal formation2011
Author(s)
Itoh Y, Yoshimura M, Niimi K, Usami M, Hamamoto S, Kobayashi T, Hirose M, Okada A, Yasui T, Tozawa K, Kohri K
-
Journal Title
Archivio Italiano di Urologia e Andrologia
Volume: 83
Pages: 23-5
Peer Reviewed
-
-
[Journal Article] Crucial role of the cryptic epitope SLAYGLR within osteopontin in renal crystal formation of mice2011
Author(s)
Hamamoto S, Yasui T, Okada A, Hirose M Matsui Y, Kon S, Sakai F, Kojima Y, Hayashi Y, Tozawa K, Ueda T, Kohri K
-
Journal Title
Bone and Mineral Research
Volume: 26
Pages: 2967-2977
Peer Reviewed
-
-
-
[Journal Article] Renal macrophage migration and crystal phagocytosis via inflammatory-related gene expression during kidney stone formation and elimination in mice : Detection by association analysis of stone-related gene expression and microstructural observation2010
Author(s)
Okada A, Yasui T, Fujii Y, Niimi K, Hamamoto S, Hirose M, Kojima Y, Itoh Y, Tozawa K, Hayashi Y, Kohri K
-
Journal Title
Journal of Bone and Mineral Research
Volume: 25
Pages: 2701-2711
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Calcium oxalate crystals could be englobed by macrophages during kidney stone formation in vitro and in vivo models, Renal macrophage migration and crystal phagocytosis via inflammatory-related gene expression during kidney stone formation and elimination2011
Author(s)
Okada A, Taguchi K, Hirose Y, Niimi K, Fujii Y, Kobayashi T, Usami M, Hamamoto S, Hirose M, Itoh Y, Yasui T, Tozawa K, Kohri K, Okada A, Yasui T, Ichikawa J, Nakaoka K, Hirose Y, Taguchi K, Niimi K, Fujii Y, Usami M, Kobayashi T, Ando R, Hamamoto S, Hiros
Organizer
European Association of Urology Annual Congress 2012
Place of Presentation
Paris(France), Washington(U. S. A)
Year and Date
20110514-20120228
-
-
-
[Presentation] Renal macrophage migration and crystal phagocytosis via inflammatory-related gene expression during kidney stone formation and elimination in mice2011
Author(s)
Okada A, Hamamoto S, Ichikawa J, Nakaoka K, Hirose Y, Taguchi K, Fujii Y, Niimi K, Usami M, Kobayashi T, Hamamoto S, Hirose M, Itoh Y, Yasui T, Tozawa K, Kohri K
Organizer
26th Annual EAU(European Association of Urology) Congress
Place of Presentation
Vienna(Austria)
Year and Date
20110318-22
-
[Presentation] Hypothetical new aspects of human kidney stone formation ; comparison of clinical and pathological findings of stone-forming and non-stone-forming kidneys2011
Author(s)
Okada A, Nakaoka K, Ichikawa J, Hirose Y, Taguchi K, Niimi K, Fujii Y, Usami M, Kobayashi T, Hamamoto S, Hirose M, Itoh Y, Yasui T, Tozawa K, Kohri K
Organizer
26th Annual EAU(European Association of Urology) Congress
Place of Presentation
Vienna(Austria)
Year and Date
20110318-22
-
[Presentation] Osteopontin has a crucial role in ultrastructural conversion of calcium oxalate crystals into matrix-involving kidney stones2011
Author(s)
Okada A, Hamamoto S, Ichikawa J, Nakaoka K, Taguchi K, Hirose Y, Fujii Y, Niimi K, Usami M, Kobayashi T, Hirose M, Itoh Y, Yasui T, Tozawa K, Kohri K
Organizer
26th Annual EAU(European Association of Urology) Congress
Place of Presentation
Vienna(Austria)
Year and Date
20110318-22
-
-
-
[Presentation] Osteopontin has a crucial role in ultrastructural conversion of calcium oxalate crystals into matrix-involving kidney stones2010
Author(s)
Okada A, Taguchi K, Nakaoka K, Hirose Y, Fujii Y, Niimi K, Kobayashi T, Hamamoto S, Hirose M, Itoh Y, Yasui T, Tozawa K, Kohri K
Organizer
FASEB Summer Research Conferences ; Osteopontin Biology
Place of Presentation
Steamboat Springs, Colorado, U. S. A.
Year and Date
20100801-06
-
-
-
-
[Presentation] Genome-wide analysis of genes related to kidney stone formation and elimination in the calcium oxalate nephrolithiasis model mouse : detection of involvement of macrophage activity2009
Author(s)
Okada A, Niimi K, Fujii Y, Kobayashi T, Usami M, Hamamoto S, Hirose M, Yasui T, Tozawa K, Kohri K
Organizer
30th Congress of the Societe International d' Urologie
Place of Presentation
Shanghai, China
Year and Date
20091101-05
-
[Presentation] Osteopontin has an important role in kidney stone formation and elimination via renal macrophage recruitment and activation2009
Author(s)
Okada A, Tozawa K, Niimi K, Hirose Y, Kobayashi T, Usami M, Hamamoto S, Hirose M, Itoh Y, Yasui T, Hayashi Y, Kohri K
Organizer
AUA 2009 Annual Meeting
Place of Presentation
Chicago(USA)
Year and Date
20090425-30
-
-
-
-
-
