2010 Fiscal Year Final Research Report
Roles of branched-type disialyl-glycolipid antigen in renal cell cancer
| Project/Area Number |
21791534
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | The Noguchi Institute |
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Principal Investigator |
TSUCHIDA Akiko The Noguchi Institute, 研究部, 研究員 (70378024)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 腎癌 / 糖脂質 / 糖転移酵素 / ジシアリル糖鎖 / 腫瘍マーカー |
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| Research Abstract |
It has been reported that novel antigen GalNAc-disialyl Lc4 (GalNAc-DSLc4) is associated with malignant features of renal cancers. The stable transfectant of GalNAc-T2 cDNA using TUHR14TKB cells, resulting in the high expression of GalNAc-DSLc4, was established to analyze their phenotypes. To date, specific adhesion of transfectants onto laminin-coating plates among those with various extracellular matrices was observed by the dynamic monitoring using real-time cell electronic sensing (RT-CES). To explain the specific adhesion of GalNAc-DSLc4 transfectants on the laminin-coating plate, we investigated various adhesion molecules such as integrins known to interact with laminin. And recently, we revealed the localization of integrin β1 and GalNAc-DSLc4 antigen on the cell surface by immunofluorescence assay with confocal laser microscopy.
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