Development and analysis of a mouse model of preeclampsia using transient in vivo gene transfer system

2010 Fiscal Year Final Research Report

• Project/Area Number: 21791553
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Osaka University
• Principal Investigator: KOYAMA Shinsuke Osaka University, 医学系研究科, 助教 (30528005)
• Project Period (FY): 2009 – 2010
• Keywords: 妊娠高血圧症候群 / 生体内遺伝子導入 / マウスモデル / ex vivo

Research Abstract

Recently, "Two Step Theory" has received a lot of attention as pathological condition of preeclampsia, concretely speaking, reduced placental perfusion due to insufficient chorionic invasion to the uterus at early pregnancy is postulated as the root cause and maternal systemic endothelial dysfunction is induced by some over-expressed inhibitor of angiogenesis factor due to hypoxic status at placenta. In this research, we transferred TIMP-3 which is known as related factor of regulation of chorionic invasion to the decidua into the uterine cavity during implantation window, thus we successfully developed a mouse of preeclampsia.

Research Products

• [Journal Article] Pseudomyxoma peritonei originating from colorectal cancer during pregnancy. (2010)
  • Author(s): Koyama, S., Tomimatsu, T., Sawada, K., Kanagawa, T., Tsutsui, T., Kimura, T.
  • Journal Title: J Obstet Gynaecol Res 37(3) Pages: 254-258
• [Journal Article] Cervical insufficiency following cesarean delivery after prolonged second stage of labor : experiences of two cases. (2010)
  • Author(s): Koyama, S., Tomimatsu, T., Kanagawa, T., Sawada, K., Tsutsui, T., Kimura, T.
  • Journal Title: J Obstet Gynaecol Res 36(2) Pages: 411-413
• [Journal Article] Prenatal diagnosis of polysplenia syndrome associated with increased nuchal translucency. (2010)
  • Author(s): Koyama, S., Tomimatsu, T., Kanagawa, T., Morimoto, A., Kimura, T.
  • Journal Title: Prenat Diagn 30(10) Pages: 1005-1006
• [Journal Article] Spinal subarachnoid hematoma following spinal anesthesia in a patient with HELLP syndrome. (2010)
  • Author(s): Koyama, S., Tomimatsu, T., Kanagawa, T., Sawada, K., Tsutsui, T., KimuS., Murata, Y.
  • Journal Title: Int J Obstet Anesth 19(1) Pages: 87-91
• [Journal Article] A case of complete hydatidiform mole with coexistent fetus: conclusive diagnosis of androgenesis of the molar placenta by variation of paternal acrocentric short arms. (2010)
  • Author(s): Koyama, S., Tomimatsu, T., Sawada, K., Kanagwaw Isobe, A., Kinugasa, Y., Tsutsui, T., Kimura, T.
  • Journal Title: Am J Perinatol 27(2) Pages: 143-149
• [Presentation] Bizarre expression of tissue inhibitor of metalloproteinase-3 in feto-maternal interface caused gertational hypertension in mice model. (2010)
  • Author(s): Kimura, T., Zhang, Q., Nakamura, H., Kumasawa K, Koyama S., Tabata C., Tsutsui, T.
  • Organizer: XI International Congress of Reoroductive Immunology.
  • Place of Presentation: Cairns, AUSTRALIA
  • Year and Date: 20100815-20100819
• [Presentation] 中隔子宮を有する挙児希望患者に対する子宮鏡下中隔子宮切除述後の妊娠予後 (2010)
  • Author(s): 筒井建紀, 香山晋輔, 田畑知沙, 横井猛, 木村正, 朝倉寛之
  • Organizer: 第50回日本産婦人科内視鏡学会学術講演会
  • Place of Presentation: 東京都(京王プラサセホテル)
  • Year and Date: 20100729-20100931
• [Presentation] Mayer-Rokitansky-Kuster-Hauser syndromeに対して施行した腹腔鏡補助下造膣術(Cavydov変法)的検討 (2010)
  • Author(s): 瀧内剛, 香山晋輔, 横井猛, 金山智子, 筒井建紀, 木村正
  • Organizer: 第50回日本産婦人科内視鏡学会学術講演会
  • Place of Presentation: 東京都(京王プラサセホテル)
  • Year and Date: 20100729-20100731
• [Presentation] Significance of feto-maternal interface : from implantation failure to preeclampsia in model mice. (2010)
  • Author(s): Kimura, T., Zhang, Q., Nakamura, H., Kumasawa, K., Koyama, S., Tabata, C., Tsutsui, T.
  • Organizer: 2nd International Conference on Reproductive Immunology.
  • Place of Presentation: Shanghai(招待講演)
  • Year and Date: 2010-10-02
• [Presentation] 胎児頭蓋内出血を来した特発性血小板減少性紫斑病(Idiopathic Thrombocytopen ic Purpura : ITP)合併妊娠の一症例 (2010)
  • Author(s): 香山晋輔, 朋和, 谷口友基子, 晶磯, 金川武司, 澤田健二郎, 富松拓治, 木村正
  • Organizer: 第62回日本産婦人科学会学術講演会
  • Place of Presentation: 東京都(東京國際フォーラム)
  • Year and Date: 2010-10-02