Role of ESCRT proteins in the malignant phenotype control of Head and Neck Cancer Cells

2010 Fiscal Year Final Research Report

• Project/Area Number: 21791589
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Otorhinolaryngology
• Research Institution: Tohoku University
• Principal Investigator: KATO Kengo Tohoku University, 病院, 助教 (40455788)
• Co-Investigator(Renkei-kenkyūsha): TANAKA Nobuyuki 宮城県立がんセンター(研究所), 免疫学部, 部長 (60280872)
• Project Period (FY): 2009 – 2010
• Keywords: 酵素 / 蛋白質 / ユビキチン / 小胞輸送

Research Abstract

Head and Neck Cancer (HNC) originates from the pharynx, and larynx. Improvement in the management, as well as targeted therapy, is required to maintain patients' quality of life and survival. In this study, we investigated how HNC which is linked to EMT is regulated by the ESCRT vesicular transport system. Forced expression of the dominant negative ESCRT regulator, the dominant negative VPS4, in HNC cell lines reduced the cancer stemness phenotype, such as ALDH1 activity. In contrast, cell morphology and motility was not altered. Our results suggest that ESCRT system may control the malignant phenotypes of HNC.

Research Products

• [Journal Article] Exosome secretion of dendritic cells is regulated by Hrs, an ESCRT-0 protein. (2010)
  • Author(s): Tamai K, Tanaka N, Nakano T, Kakazu E, Kondo Y, Inoue J, Shiina M, Fukushima K, Hoshino T, Sano K, Ueno Y, Shimosegawa T, Sugamura K.
  • Journal Title: Biochem Biophys Res Commun 399 Pages: 384-390
  • Peer Reviewed
• [Journal Article] Regulation of interleukin-21 receptor expression and its signal transduction by WSB-2. (2010)
  • Author(s): Nara H, Onoda T, Rahman M, Araki A, Juliana FM, Tanaka N, Asano H.
  • Journal Title: Biochem Biophy Res Commun 392 Pages: 171-177
  • Peer Reviewed
• [Journal Article] Identification of a high incidence region for retroviral vector integration near exon 1 of the LMO2 locus. (2009)
  • Author(s): Yamada K, Tsukahara T, Yoshino K, Kojima K, Agawa H, Yamashita Y, Amano Y, Hatta M, Matsuzaki Y, Kurotori N, Wakui K, Fukushima Y, Osada R, Shiozawa T, Sakashita K, Koike K, Kumaki S, Tanaka N, Takeshita T.
  • Journal Title: Retrovirology 6 Pages: 79
  • Peer Reviewed
• [Presentation] 間接熱量計による頭頸部癌化学放射線療法の安静時エネルギー消費量(REE)の測定 (2011)
  • Author(s): 加藤健吾
  • Organizer: 第26回日本静脈経腸栄養学会
  • Place of Presentation: 名古屋市
  • Year and Date: 2011-02-18
• [Presentation] 間接熱量計による頭頸部癌化学放射線療法の安静時エネルギー消費量(REE)の測定 (2010)
  • Author(s): 加藤健吾
  • Organizer: 第48回日本癌治療学会学術集会
  • Place of Presentation: 京都府
  • Year and Date: 2010-10-28
• [Presentation] TPF同時併用化学放射線療法に関連した間質性肺炎の検討 (2010)
  • Author(s): 加藤健吾
  • Organizer: 第34回日本頭頸部癌学会
  • Place of Presentation: 東京
  • Year and Date: 2010-06-11
• [Remarks] ホームページ等