Basical study for regulation of apoptosis; Aiming to improve the results and to enlarge the application of cochlear implant surgery.

2010 Fiscal Year Final Research Report

• Project/Area Number: 21791597
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Otorhinolaryngology
• Research Institution: The University of Tokyo
• Principal Investigator: KASHIO Akinori The University of Tokyo, 医学部附属病院, 助教 (20451809)
• Project Period (FY): 2009 – 2010
• Keywords: アポトーシス / 人工内耳 / ウロキナーゼ / タンパク治療

Research Abstract

Urokinase-type plasminogen activator (uPA) and its receptor (uPAR) are recently shown to have close relationship to the apoptosis. We demonstrated that uPAR was in the cochlea especially in organ of corti and spiral ganglion, which is indicating that the uPA-uPAR anti-apoptotic system is working in cochlea. Next we demonstrated, using cochlear organotypic cultures of the rat, that uPA attenuates the aminoglicoside induced apoptotic hair cell death in a dose dependent manner. We have also demonstrated that artificial anti-apototic protein PTD-FNK attenuates the aminoglicoside induced apoptotic hair cell death by affecting caspase-9 related mitochondrial apoptotic pathway.

Research Products

• [Journal Article] Carhart's notch, a 2kHz bone conduction threshold dip, is not a definitive predictor of stapes fixation in conductive hearing loss accompanied with a normal ear drum. (2011)
  • Author(s): Kashio A, Ito K, Kakigi A, Karino S, Iwasaki S, Sakamoto T, Yasui T, Suzuki M, Yamasoa T.
  • Journal Title: Arch Otolaryngol Head Neck Surg 137(3) Pages: 236-240
  • Peer Reviewed
• [Journal Article] Preservation of vestibular function after scala vestibuli cochlear implantation. (2011)
  • Author(s): Suzuki M, Goto T, Kashio A, Yasui T, Sakamoto T, Ito K, Yamasoba T.
  • Journal Title: Auris Nasus Larynx. 38(5) Pages: 638-642
  • Peer Reviewed
• [Journal Article] Evaluation of the Carhart effect in congenital middle ear malformation with both an intact external ear canal and a mobile stapes footplate. (2011)
  • Author(s): Sakamoto T, Kakigi A, Kashio A, Kanaya K, Suzuki M, Yamasoba T.
  • Journal Title: ORL J Otorhinolaryngol Relat Spec. 73(2) Pages: 61-67
  • Peer Reviewed
• [Journal Article] Hydorgen in drinking water attenuates noise-induced hearing loss in guinea pigs Neurosci Lett (2011)
  • Author(s): Lin Y, Kashio A, Sakamoto T, Suzukawa K, Kakigi A, Yamasoba T
  • Journal Title: 487(1) Pages: 12-16
  • Peer Reviewed
• [Journal Article] Dislocation of stapes with footplate fracture caused by indirect trauma. (2010)
  • Author(s): Kagoya R, Ito K, Kashio A, Karino S, Yamasoba T.
  • Journal Title: Ann Otol Rhinol Laryngol. 119(9) Pages: 628-630
  • Peer Reviewed
• [Journal Article] Effect of Burow's solution on the guinea pig inner ear. (2010)
  • Author(s): Suzuki M, Kashio A, Sakamoto T, Yamasoba T.
  • Journal Title: Ann Otol Rhinol Laryngol. 119(7) Pages: 495-500
  • Peer Reviewed
• [Journal Article] A spontaneous true aneurysm of the superficial temporal artery treated by surgical resection. (2010)
  • Author(s): Sakamoto T, Sugimoto M, Kakigi A, Iwamura H, Kashio A, Suzuki M, Yamasoba T
  • Journal Title: Auris Nasus Larynx.
  • Peer Reviewed
• [Journal Article] Cochlear implantation in a patient with atypical Cogan's syndrome complicated with hypertrophic cranial pachymeningitis. (2010)
  • Author(s): Kawamura S, Sakamoto T, Kashio A, Kakigi A, Ito K, Suzuki M, Yamasoba T.
  • Journal Title: Auris Nasus Larynx.
  • Peer Reviewed
• [Journal Article] Comparison of hearing improvement and complications after stapes surgery with and without potassium titanyl phosphate laser for manipulation of the foot plate. (2010)
  • Author(s): Sakamoto T, Iwamura H, Kashio A, Ito K, Kaga K, Suzuki M, Yamasoba T.
  • Journal Title: ORL J Otorhinolaryngol Relat Spec. 72(1) Pages: 16-21
  • Peer Reviewed
• [Journal Article] Effect of vitamin C depletion on age-related hearing loss in SMP30/GNL knockout mice. (2009)
  • Author(s): Kashio A, Amano A, Kondo Y, Sakamoto T, Iwamura H, Suzuki M, Ishigami A, Yamasoba T.
  • Journal Title: Biochem Biophys Res Commun. 390(3) Pages: 394-398
  • Peer Reviewed
• [Journal Article] Ocular vestibular-evoked myogenic potentials (oVEMPs) require extraocular muscles but not facial or cochlear nerve activity. (2009)
  • Author(s): Chihara Y, Iwasaki S, Ushio M, Fujimoto C, Kashio A, Kondo K, Ito K, Asakage T, Yamasoba T, Kaga K, Murofushi T.
  • Journal Title: Clin Neurophysiol. 120(3) Pages: 581-587
  • Peer Reviewed
• [Journal Article] Cochlear implantation in children with inner ear malformations : Proceedings of the 7th Asia Pacific Symposium on Cochlear Implants and Related Sciences -
  • Author(s): Kashio A, Ogata E, Akamatsu Y, Ito K, Kaga K, Yamasoba T
  • Journal Title: APSCI on press.
• [Presentation] Urokinase-type plasminogen activator attenuates hair cell damage induced by aminoglycoside.
  • Organizer: ARO 2011 Baltimore
• [Presentation] Round Window administration of TAT-FNK protein, a Bcl-XL derivative,immersed in gelatin sponge prevents
  • Organizer: aminoglycoside-induced cochlear damage ARO 2010 Los Angels
• [Remarks] ホームページ等