2010 Fiscal Year Final Research Report
Triobp, the novel deafness gene-stereocilia rootlet and hearing
| Project/Area Number |
21791615
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Kyoto University |
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Principal Investigator |
KITAJIRI Shinichiro Kyoto University, 医学研究科, 助教 (00532970)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 内耳 / 遺伝性難聴 / 不動毛 / アクチン |
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| Research Abstract |
Knock out mice (KO mice) of Triobp isoforms are generated. It turned out that Triobp-4/5 KO stereocilia lack rootlet, and Triobp-1/5 KO mice are embryonic lethal. Both of these two models are deficient in two isoforms respectively, but single isoform specific KO mice are now generating. Adenovirus vector to express Triobp isoforms were also generated. Plasmids to express Triobp fragments were transfected into cultured cell to examine domain structure of Triobp.
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