2011 Fiscal Year Final Research Report
Spatiotemporal expression of CTP, a novel biomarker of perilymphatic fistula, in the perilymph of rats.
| Project/Area Number |
21791651
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Saitama Medical University (2011)
Nippon Medical School (2009-2010) |
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Principal Investigator |
SHINDO Susumu 埼玉医科大学, 医学部, 講師 (00350067)
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| Project Period (FY) |
2009 – 2011
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| Keywords | perilymphatic fistula / CTP / inner ear |
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| Research Abstract |
The COCH gene mutated in DFNA9, an autosomal dominant hereditary sensorineural hearing loss and vestibular disorder, encodes Cochlin. Previously, we reported three bovine Cochlin isoforms, p63s, p44s, and p40s, which exhibit significant molecular heterogeneity in vivo. We have characterized Cochlin isoforms by generating four isoform-specific anti-Cochlin antibodies. The same three Cochlin isoforms, p63s, p44s, and p40s, were detected in human and cow inner ear tissue ; however, p44s and p40s were not detected in perilymph. We identified a novel short 16kDa isoform in human perilymph and a 18-23kDa isoform in cow perilymph, named Cochlin-tomoprotein(CTP), corresponding to the N-terminus of full-length Cochlin(p63s) and the LCCL domain. Notably, CTP contains all of the known mutation sites associated with DFNA9.
In this study, we investigated the expression of cochlin isoforms in the inner ear tissues and perilymph during postnatal development of rats. By western blotting, no expression of CTP was detected in the inner ear tissues, and was increased in the perilymph during postnatal development. The expression of p63s was increased in the inner tissues, although expression of p63s was decreased in the perilymph during postnatal development. These results may suggest an important clue of The pathogenesis of DFNA9 is not fully clarified as yet, and this novel perilymph-associated CTP isoform might provide mechanistic clues to how mutations in the COCH gene damage the inner ear function.
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