Restore vision by transgene of Channelrhodopsin2 on ON-type bipolar cells in genetically blind rat.

2010 Fiscal Year Final Research Report

• Project/Area Number: 21791664
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Tohoku University
• Principal Investigator: SUGANO Eriko Tohoku University, 国際高等研究教育機構, 助教 (70375210)
• Project Period (FY): 2009 – 2010
• Keywords: 神経再生 / 網膜 / 遺伝子導入 / ウイルス / プロモーター

Research Abstract

I constructed pAAV-Glu6-ChR2 vector that induce ChR2 gene by the metabotropic glutamate receptor (mGlu6) promoter into the on-type bipolar cells. I established the protocol to purify AAV by HPLC. These results showed the same induction efficiency between injection routes and serotypes of AAV.

Research Products

• [Journal Article] Differentiation of neuronal cells from NIH/3T3 fibroblasts under defined conditions. (2011)
  • Author(s): Wang Z, Sugano E, Isago H, Hiroi T, Tmai M, Tomita H.
  • Journal Title: Development Growth and Differentiation In press
  • Peer Reviewed
• [Journal Article] Immune responses to adeno-associated virus type 2 encoding channelrhodopsin-2 in a genetically blind rat model for gene therapy. (2011)
  • Author(s): Sugano E, Isago H, Wang Z, Murayama N, Tamai M, Tomita H.
  • Journal Title: Gene Therapy 18(3) Pages: 266-274
  • Peer Reviewed
• [Journal Article] Dissecting a role for melanopsin in behavioural light aversion reveals a response independent of conventional photoreception. (2010)
  • Author(s): Semo M, Gias C, Ahmado A, Sugano E, Allen AE, Lawrence JM, Tomita H, Coffey PJ, Vugler AA.
  • Journal Title: PLoS One 29;5(11) Pages: e15009
  • Peer Reviewed
• [Presentation] チャネルロドプシンを用いた視覚再生により得られる視覚の特性と予想される視覚情報について (2011)
  • Author(s): 富田浩史, 菅野江里子, 砂金ひとみ, 王卓, 村山なみえ, 玉井信
  • Organizer: 日本視覚学会
  • Place of Presentation: 工学院大学(東京都)
  • Year and Date: 2011-01-20
• [Presentation] Optimization of volvox-derived channelrhodopsin-1 to express in mammalian cells. (2010)
  • Author(s): Sugano, E., Tomita, H., Isago, H., Hiroi, T., Wang, Z., Tamai, M.
  • Organizer: XIVth International Symposium on Retinal Degeneration.
  • Place of Presentation: Mont-Tremblant(Quebec, Canada)
  • Year and Date: 20100714-20100716
• [Presentation] 緑藻類由来遺伝子を用いた視覚再生技術 (2010)
  • Author(s): 富田浩史, 菅野江里子, 砂金ひとみ, 王卓, 村山なみえ, 玉井信
  • Organizer: 日本人工臓器学会
  • Place of Presentation: 国際センター(仙台)
  • Year and Date: 2010-11-20
• [Presentation] チャネルロドプシン-2を用いた視覚再生の可能性 (2010)
  • Author(s): 菅野江里子
  • Organizer: 第6回網脈絡膜変性フォーラム
  • Place of Presentation: いわて県民情報交流センター(盛岡)
  • Year and Date: 2010-10-10
• [Presentation] NIH 3T3線維芽細胞からの神経細胞の誘導 (2010)
  • Author(s): 王卓, 菅野江里子, 砂金ひとみ, 廣井照, 玉井信, 富田浩史
  • Organizer: 日本動物学会
  • Place of Presentation: 東京大学(東京)
  • Year and Date: 2010-09-25
• [Presentation] ボルボックス由来チャネルロドプシン2を導入した遺伝盲ラットの光反応性 (2010)
  • Author(s): 砂金ひとみ, 菅野江里子, 廣井照, 王卓, 玉井信, 富田浩史
  • Organizer: 日本動物学会
  • Place of Presentation: 東京大学(東京)
  • Year and Date: 2010-09-23
• [Presentation] ボルボックス由来チャネルロドプシン-2の網膜における光特性解析 (2010)
  • Author(s): 菅野江里子、砂金ひとみ、王紅霞、王卓、廣井照、玉井信、富田浩史
  • Organizer: 平成22年度「包括脳ネットワーク」夏のワークショップ
  • Place of Presentation: 旧北海道厚生年金会館(札幌)
  • Year and Date: 2010-07-27
• [Presentation] 視機能再建のための遺伝子治療:現状と課題 (2010)
  • Author(s): 富田浩史、菅野江里子
  • Organizer: JRPS群馬県支部第11回定期総会
  • Place of Presentation: 群馬県社会福祉総合センター(前橋市)
  • Year and Date: 2010-06-19
• [Presentation] Visual Responses of Royal College of Surgeons Rats Transferred Modified Volvox Channelrhodopsin-2 Gene. (2010)
  • Author(s): Sugano, E., Tomita, H., Isago, H., Hiroi, T., Wang, Z., Tamai, M.
  • Organizer: Annual meeting Association for Research in Vision and Ophthalmology.
  • Place of Presentation: Convention Center (Fortlauderdale, FL, US)
  • Year and Date: 2010-05-04
• [Remarks] ホームページ等
  • URL: http://www.visual-neuroscience.med.tohoku.ac.jp/
• [Patent(Industrial Property Rights)] (2010)
  • Inventor(s): 富田浩史、菅野江里子
  • Industrial Property Rights Holder: 富田浩史、菅野江里子
  • Industrial Property Number: PCT,JP2010/063786
  • Filing Date: 2010-07-10