Expression analysis of microRNA during development of murine experimental autoimmune uveoretinitis

2010 Fiscal Year Final Research Report

• Project/Area Number: 21791681
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Osaka University
• Principal Investigator: HASHIDA Noriyasu Osaka University, 医学部附属病院, 医員 (30456959)
• Project Period (FY): 2009 – 2010
• Keywords: 眼免疫学 / リポソーム / ドラッグデリバリーシステム

Research Abstract

We investigated sequential expression changes in microRNA (miRNA) during the natural course of murine experimental autoimmune uveoretinitis (EAU). Dynamic expression changes of miRNA during EAU were observed from the start of immunization. The expression of most miRNAs changed dramatically from the start of immunization. To search for miRNA that might regulate the inflammatory genes, we examined the potential target genes that might control ocular inflammation in EAU. As a result, up-regulated miR-381 and miR-466b-3-3p expressions, a candidate regulator of the NF-kappaB inhibitor (NFKBIA), down-regulation of miR-322* and miR-465b-5p expression, a regulator of NF-kappaB (NFKBA), occurred on days 5, 15and 20. And also, down-regulation of let-7b* and miR-495, a regulator of IL-10 occurred on days 20 and 30. Dynamic expression changes in miRNAs may be involved in EAU pathogenesis. Our results suggested that changes in the expression levels of specific miRNAs and their candidate target genes are involved in development and remission of EAU.

Research Products

• [Journal Article] Fuchs' heterochromic iridocyclitis associated with ocular toxocariasis. (2011)
  • Author(s): Hashida N, Ohguro N, Maruyama H.
  • Journal Title: Jpn J Ophthalmol. 55 Pages: 76-78
  • Peer Reviewed
• [Journal Article] Anterior segment optical coherence tomography findings of presumed intraocular tuberculosis. (2011)
  • Author(s): Hashida N, Terubayashi A, Ohguro N.
  • Journal Title: Cutan Ocul Toxicol. 30 Pages: 75-77
  • Peer Reviewed
• [Journal Article] Comprehensive gene-expression profile in murine oxygen-induced retinopathy. (2009)
  • Author(s): Sato T, Kusaka S, Hashida N, Saishin Y,Fujikado T, Tano Y.
  • Journal Title: Br J Ophthalmol 93 Pages: 96-103
  • Peer Reviewed
• [Journal Article] Case of optic neuritis associated with cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL) (2009)
  • Author(s): Hashida N, Ito S, Hasegawa T, Kajita T,Morifuji H, Yamazaki Y, Sakagami K,Yokotani S, Tanaka T, Endoh B, Egawa N, Inoue H, Tomimoto H, Takahashi R.
  • Journal Title: Nippon Ganka Gakkai Zasshi 113 Pages: 505-512
  • Peer Reviewed
• [Journal Article] Ultrastructure appearance of iris flocculi in association with the thoracic aortic aneurysm and dissections. (2009)
  • Author(s): Hashida N, Ohguro N, Morimoto Y, Oiki E, Morisaki H, Morisaki T, Tano Y.
  • Journal Title: Br J Ophthalmol 93 Pages: 1409-1410
  • Peer Reviewed
• [Presentation] Expression analysis of cytokine and chemokine genes during the natural course of murine experimental autoimmune uveoretinitis. (2010)
  • Author(s): Hashida N, Ohguro N, Houki S, Nishida K.
  • Organizer: ARVO 2011 Annual meeting
  • Place of Presentation: Fort Lauderdale, USA.
  • Year and Date: 2010-05-03
• [Presentation] 日本眼炎症学会学術奨励賞受賞講演(炎症部位集積性リポソームを用いたドラッグデリバリーシステムの開発) (2009)
  • Author(s): 橋田徳康
  • Organizer: 第43回日本眼炎症学会
  • Place of Presentation: 大阪市
  • Year and Date: 2009-07-11
• [Presentation] Efficacy and Complication of Intravitreal Rituximab Injection for treating Primary Intraocular Lymphoma. (2009)
  • Author(s): Yamazaki Y, Hashida N, Saishin Y, Ohguro N, Tano Y.
  • Organizer: The 10th International Ocular Inflammation Society Congress
  • Place of Presentation: Prague, Czech republic
  • Year and Date: 2009-06-01
• [Book] 眼科プラクティス28.眼感染症の謎を解くII.眼感染症事典6.網脈絡膜炎3)CMV (2009)
  • Author(s): 橋田徳康
  • Total Pages: 194-195
  • Publisher: 文光堂