2010 Fiscal Year Final Research Report
Fibroblast Aggregates Grown in Non-adhesive Culture Obtain Hair Inductive Ability
| Project/Area Number |
21791753
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Plastic surgery
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| Research Institution | Keio University |
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Principal Investigator |
SHIMIZU Ruka Keio University, 医学部, 研究員 (50445392)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 毛包再生 / 非接着培養 / 線維芽細胞 |
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| Research Abstract |
Interactions between epithelial and dermal cells are essential for hair follicle morphogenesis and maintenance. In experimental trials of hair regeneration, isolated dermal cells have been shown to possess hair-inducing capacity. However, dermal cells lose this potential immediately after cultivation. Sphere-forming multipotent cells derived from the dermis possess hair-inducing capacity. These previous findings raise the possibility that hair-inducing capacity depends on the process of sphere-formation. To address this issue, we compared the in vitro and in vivo characteristics of two-dimensionally cultured or thereafter sphere formation-induced dermal cells from fetal mice and human, and lung cells from adult mice. Sphere-forming cells but not two-dimensionally cultured cells possessed in vivo hair-inducing capacity.
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