cytological evaluation on different effect of parathyroid hormone (PTH) in osteoblasts and preosteoblasts

2010 Fiscal Year Final Research Report

• Project/Area Number: 21791784
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphological basic dentistry
• Research Institution: Hokkaido University
• Principal Investigator: LI Minqi Hokkaido University, 大学院・歯学研究科, 助教 (60447612)
• Project Period (FY): 2009 – 2010
• Keywords: 解剖学 / 細胞・組織 / 遺伝子 / 歯学 / 骨

Research Abstract

PTH administration led to significant increases in the pre-osteoblastic proliferation in both the wild-type and c-fos^<-/-> mice. The pre-osteoblasts present on the bone surfaces did not seem to be actively producing bone matrix in c-fos^<-/-> mice. We concluded that the absence of osteoclasts might hinder PTH-driven bone anabolism and that osteoclastic presence may be necessary for full osteoblastic differentiation and enhanced bone formation after the intermittent PTH administration.

Research Products

• [Journal Article] FGFR3 down-regulates PTH/PTHrP receptor gene expression by mediating JAK/STAT signaling in chondrocytic cell line. (2010)
  • Author(s): Li M, Seki Y, Freitas HLP, Nagata M, Kojima T, Sultana S, Ubaidus S, Maeda T, Shimomura-Kuroki J, Henderson J, Tamura M, Liu Z, Guo Y, Yamamoto T, Oda K, Takagi R, Amizuka N.
  • Journal Title: J Electron Microsc. 59(3) Pages: 227-236
  • Peer Reviewed
• [Journal Article] Ultrastructural observation on cells with characteristics of histological criteria for preosteoblasts in mouse tibial metaphysis. (2010)
  • Author(s): Narimatsu K, Li M, FreitaS PHL, Sultana S, Ubaidus S, Kojima T, Liu Z, Guo Y, Suzuki R Yamamoto T, Oda K, Amizuka N.
  • Journal Title: J Electron Microsc. 59(5) Pages: 427-436
  • Peer Reviewed
• [Journal Article] Immunolocalization of DMP-1 and sclerostin in the epiphyseal trabecule and diaphyseal cortical bone of osteoprotegerin deficient mice. (2010)
  • Author(s): Masuki H, Li M, Guo Y, Hasegawa T, Liu Z, Suzuki R, Oda K, Yamamoto T, Kawanami M, Amizuka N
  • Journal Title: Biomedical Res. 31(5) Pages: 307-318
  • Peer Reviewed
• [Journal Article] Ultrastructural Assessment of Mineral Crystallization and Collagen Mineralization in Bone. (2010)
  • Author(s): Li M, Hasegawa T, Masuki H, Liu Z, Guo Y, Suzuki R, Yamamoto T, Freitas PHL, Amizuka N
  • Journal Title: J.Oral Biosci. 52(2) Pages: 94-99
  • Peer Reviewed
• [Journal Article] 骨・軟骨に対するPTH/PTHrP作用における形態学的知見Clin Calcium (2009)
  • Author(s): 網塚憲生、李敏啓、田村正人、織田公光
  • Journal Title: 医薬ジャーネル社 19 Pages: 935-943
• [Journal Article] Intermittent PTH administration stimulates pre-osteoblastic proliferation without leading to enhanced bone formation in osteoclast-less c-fos (-/-) mice. (2009)
  • Author(s): Freitas PHL, Li M, Ninomiya T, Nakamura M, Ubaidus S, Oda K, Udagawa N, Maeda T, Takagi R, Amizuka N
  • Journal Title: J Bone Miner. Res. 24(9) Pages: 1586-1597
  • Peer Reviewed
• [Journal Article] FGF23 is mainly synthesized by osteocytes in the regularly distributed osteocytic lacunar canalicular system established after physiological bone remodeling. (2009)
  • Author(s): Ubaidus S, Li M, Sultana S, de Freitas PH, Oda K, Maeda T, Takagi R, Amizuka N.
  • Journal Title: J Electron Microsc. 58(6) Pages: 381-392
  • Peer Reviewed
• [Presentation] Histochemical assessment of altered bone tissue in transgenic mice overexpressing parathyroid hormone-related peptide (PTHrP) driven by type I collagen promoter. (2010)
  • Author(s): Li M, Hasegawa T, Nakamura S, Sasaki M, Liu Z, Guo Y, Suzuki R, Yamamoto T, Amizuka N
  • Organizer: The American Society for Bone and Mineral Research (ASBMR), Metro Toronto Convention Centre
  • Place of Presentation: South Building Toronto, ON, Canada
  • Year and Date: 2010-10-17
• [Presentation] 骨芽細胞特異的PTHrPトランジェニックマウスの骨組織における解析 (2010)
  • Author(s): 李敏啓、長谷川智香、柳鋳晟、増木英郎、佐々木宗輝、田幡千尋、小守壽文、山本恒之、網塚憲生
  • Organizer: 第52回歯科基礎医学会
  • Place of Presentation: タワーホール船堀、東京
  • Year and Date: 2010-09-21
• [Presentation] 骨芽細胞特異的PTHrP過剰発現マウスの骨組織における組織化学的解析 (2010)
  • Author(s): 李敏啓、郭頴、小守壽文、網塚憲生
  • Organizer: 第28回日本骨代謝学会
  • Place of Presentation: 京王プラザホテル、東京
  • Year and Date: 2010-07-23
• [Presentation] type I collagen promoterを用いたPTHrP Tgマウスの骨芽細胞における解析 (2010)
  • Author(s): 李敏啓、長谷川智香、柳鋳晟、郭穎、鈴木礼子、沢村祥子、山本恒之、網塚憲生
  • Organizer: 第30回日本骨形態計測学会
  • Place of Presentation: 米子コンベンションセンター、鳥取
  • Year and Date: 2010-05-14
• [Presentation] Histological assessment for PTH/PTHrP signaling on osteoblasts. (2010)
  • Author(s): Amizuka N, Li M, Freitas PHL, Liu Z, Guo Y, Suzuki R, Yamamoto T
  • Organizer: 14th international congress of endocrinology
  • Place of Presentation: Kyoto International Conference Center, Kyoto, JAPAN
  • Year and Date: 2010-03-28
• [Presentation] type I collagen promoterを用いたPTHrP Tgマウスの骨芽細胞における解析 (2010)
  • Author(s): 網塚憲生、長谷川智香、中村沙也香、Paulo Freitas、柳鋳晟、郭頴、鈴木礼子、李敏啓
  • Organizer: 第22回北海道骨粗髪症研究会
  • Place of Presentation: 北海道大学医学部臨床講義棟大講堂、札幌
  • Year and Date: 2010-01-23
• [Presentation] 副甲状腺ホルモン問歓投与による前骨芽細胞の分化増殖について (2010)
  • Author(s): 網塚憲生、長谷川智香、Paulo Freitas、柳鋳晟、郭頴、鈴木礼子、李敏啓
  • Organizer: 第22回北海道骨粗鬆症研究会
  • Place of Presentation: 北海道大学医学部臨床講義棟大講堂、札幌
  • Year and Date: 2010-01-23
• [Remarks] ホームページ
  • URL: http://www.den.hokudai.ac.jp/anatomy2/hokudai_d/index.html