2010 Fiscal Year Final Research Report
Searching for environmental signals sensed by group A streptococcal two-component systems
Project/Area Number |
21791786
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Morphological basic dentistry
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Research Institution | Osaka University |
Principal Investigator |
NAKATA Masanobu Osaka University, 大学院・歯学研究科, 助教 (90444497)
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Project Period (FY) |
2009 – 2010
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Keywords | 二成分制御系 / 病原因子 / 環境応答 / 転写調節 |
Research Abstract |
Streptococcus pyogenes (group A streptococci; GAS) is a human pathogen responsible for a wide variety of diseases that ranges from self-limiting purulent infections such as pharyngitis, tonsillitis, and impetigo to severe necrotizing fasciitis and autoimmune diseases including acute rheumatic fever and glomerulonephritis. To successfully evade host immune responses and cause such diverse diseases, GAS might acclimate to environmental changes and control the expression of a variety of genes including virulence genes. In this study, deletion mutants of genes encoding both histidine kinases and response regulators were constructed in a background of a serotype M3 GAS strain. Utilizing these mutant strains and a silkworm infection model, we revealed that several two-component systems are crucial for GAS pathogenesis. Moreover, analysis of the virulence gene expression in mutant strains under several culture conditions indicated specific environmental signals are sensed by two-component systems.
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[Journal Article] Pili of oral Streptococcus sanguinis bind to salivary amylase and promote the biofilm formation.2011
Author(s)
Okahashi N, Nakata M, Terao Y, Isoda R, Sakurai A, Sumitomo T, Yamaguchi M, Kimura RK, Oiki E, Kawabata S, Ooshima T.
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Journal Title
Microb Pathog 50(3-4)
Pages: 148-154
Peer Reviewed
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[Journal Article] Pili of oral Streptococcus sanguinis bind to fibronectin and contribute to cell adhesion.2010
Author(s)
Okahashi N, Nakata M, Sakurai A, Terao Y, Hoshino T, Yamaguchi M, Isoda R, Sumitomo T, Nakano K, Kawabata S, Ooshima T.
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Journal Title
Biochem Biophys Res Commun 391(2)
Pages: 1192-1196
Peer Reviewed
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[Journal Article] Mode of expression and functional characterization of FCT-3 pilus region encoded proteins in the Streptococcus pyogenes serotype M49.2009
Author(s)
Nakata M, Koller T, Moritz K, Ribardo D, Jonas L, McIver KS, Sumitomo T, Terao Y, Kawabata S, Podbielski A, Kreikemeyer B.
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Journal Title
Infect Immun 77(1)
Pages: 32-44
Peer Reviewed
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