2010 Fiscal Year Final Research Report
Next-generation method with electrophoresis in microRNA expression profiling
| Project/Area Number |
21791819
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Asahi University |
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Principal Investigator |
HAYASHI Toru Asahi University, 歯学部, 助教 (10454266)
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| Project Period (FY) |
2009 – 2010
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| Keywords | マイクロRNA / 変性剤濃度勾配ゲル電気泳動法 |
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| Research Abstract |
The purpose of this study was to establish a new method to analyze microRNAs (miRNAs) expression profile. In denaturing gradient gel electrophoresis DNA fragments of the same length but with different base-pair sequences can be separated. This method enables us to detect miRNAs including novel sequences and to distinguish single nucleotide difference among miRNAs. Then, I attempted to detect miRNAs showing specific expression pattern among multiple samples by this electrophoresis. This method distinguished tiny sequences variation successfully, suggesting that it is probable to detect miRNA variants.
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