バイオ応用を目指した多機能ナノカーボン材料の開発

2011 Fiscal Year Annual Research Report

• Project/Area Number: 21791929
• Research Institution: Hokkaido University
• Principal Investigator: 阿部 薫明 北海道大学, 大学院・歯学研究科, 助教 (40374566)
• Keywords: 歯科用材料 / 生体適合性 / カーボンナノチューブ / 体内動態

Research Abstract

本研究では、フラーレンやカーボンナノチューブ(以下、CNT)などの炭素系ナノ微粒子(ナノカーボン)のバイオ応用の可能性を検討する為に、まずナノカーボンの親水化処理、その体内動態の可視化を試みた。CNTのバイオ応用への第一段階として、コハク酸過酸化物との反応により数百ppmの濃度で数週間に渡り沈殿を生じずに水中へと分散することに成功した。得られたCNT誘導体をマウスへと投与し経過観察を行った(最大4週間)ところ、状態観察・体重推移などから対照群との顕著な差異は見られなかった。投与後1-4週間のマウスから肺・肝臓を摘出し、透過型電子顕微鏡を用い観察したところ、各臓器へのCNT誘導体の到達・滞留が確認され、投与物質が血流に乗り各臓器へと到達している事が示唆された。また、CNTの蓄積が見られた臓器(肝臓)由来の細胞を用いて、刺激性の評価を行ったところ、著しい刺激応答は観察されなかった。今回行った細胞試験・動物実験などの結果からは、CNT誘導体の生体への顕著な毒性は見られなかった。CNTを細胞培養の足場材への応用を試み、作成した足場材へのタンパクや細胞のCNTへの吸着挙動の検討、表面形状の電子顕微鏡観察、および理論計算を行った。その結果、CNT足場材がタンパクや細胞の高い吸着能を持つことが示された。これは、グラフェンシートに比べ、CNTが曲率を持つために生ずる表面電荷の変化と、足場材を形成する際にCNTが3次元的に湾曲したマクロ構造を持つ2つの要因によるものと考えられる。また電子顕微鏡撮影のため、イオン液体を用い試料へ導電性を付与する前処理法を検討した。イオン液体塗布による前処理は乾燥プロセスを必要とせず、生物試料の構造変化を伴わない「生に近い」形態観察法として注目されている。今回は各種生物試料や、CNT足場材やフラーレンナノ構造体などナノカーボン物質の高解像度観察に成功した。

Research Products

• [Journal Article] Biodistribution of water-soluble Carbon Nanotubes in mice and its biocompatibility (2012)
  • Author(s): S.Abe, S.Itoh, D.Hayashi, T.Kobayashi, T.i Kiba, T.Akasaka, M.Uo, Y.Yawaka, S.Sato, F.Watari, T.Takada
  • Journal Title: J.Nanoscience and Nanotechnology Volume: 12 Pages: 700-706
  • DOI: 10.1166/jnn.2012.5391
  • Peer Reviewed
• [Journal Article] Interaction of Ethylene Carbonate and Graphene Chip : Density Functional Theory Study (2012)
  • Author(s): S.Abe, F.Watari, H.Tachikawa
  • Journal Title: Japanese J.Applied Physics Volume: 51 Pages: 01AH0701-03
  • DOI: DOI:10.1143/JJAP.51.01AH07
  • Peer Reviewed
• [Journal Article] Internal Distribution of Micro-/Nano-sized Inorganic Particles and their Cytocompatibility (2012)
  • Author(s): S.Abe, N.Iwadera, T.Narushima, Y.Uchida, M.Uo, T.Akasaka, Y.Yawaka, F. Watari,T.Yonezawa
  • Journal Title: J.Surface Science and Nanotechnology Volume: 10 Pages: 33-38
  • DOI: DOI:10.1143/JJAP.51.01AH07
  • Peer Reviewed
• [Journal Article] Investigation of biodistribution behavior of platinum particles in mice : Correlation between inductively coupled plasma-atomic emission spectroscopy and X-ray scanning analytical microscopy (2012)
  • Author(s): S.Abe. C.Koyama, M.Mutoh, T.Akasaka, M.Uo, F.Watari
  • Journal Title: Applied Surface Science Volume: (印刷中)(in press)
  • Peer Reviewed
• [Journal Article] Morphology and photophysical properties of one-dimensional arrayed porphyrin aggregates assisted by cyclodextrin inclusion complexation (2012)
  • Author(s): S.Abe, H.Kobayashi, T.Kiba, F.Watari, S.-I.Sato
  • Journal Title: Molecular Crystals and Liquid Crystals Volume: (accepted)
  • Peer Reviewed
• [Journal Article] Spectral narrowing of UV-visible absorption and emission spectra of anthracene-derivatives in b-cyclodextrin nanocavity : Effects of host/guest stoichiometry (2012)
  • Author(s): S.-I.Sato, Y.Yoshizumi, T.Kiba, A.Murayama, S.Abe
  • Journal Title: Molecular Crystals and Liquid Crystals Volume: (accepted)
  • Peer Reviewed
• [Journal Article] Density Functional Theory (DFT) Study on the Addition of Hydroxyl Radical (OH) to C20 (2012)
  • Author(s): T.Iyama, S.Abe, H.Tachikawa
  • Journal Title: Molecular Crystals and Liquid Crystals Volume: (accepted)
  • Peer Reviewed
• [Journal Article] Interaction between titanium and phosphoproteins revealed by chromatography column packed woth titanium beads (2012)
  • Author(s): Y.Kuboki, T.Furutake, M.Sato, Y.Sun, H.Unuma, R.Fujisawa, S.Abe, T.Akasaka, F.Watari, H.Takita, R.Sammons
  • Journal Title: Bio-Medical Materials and Engineering Volume: (accepted)
  • Peer Reviewed
• [Journal Article] Internal Distribution of Micro-/Nano-sized Inorganic Particles and their Cytocompatibility (2011)
  • Author(s): S. Abe, N. Iwadera, M. Esaki, I. Kida, M. Mutoh, T. Akasaka, M. Uo, Y. Yawaka, M. Morita, K. Haneda, T. Yonezawa, and F. Watari
  • Journal Title: Material Science and Engineering Volume: 18 Pages: 19201301-03
  • Peer Reviewed
• [Journal Article] Versatile surface modification by carbon nanotubes through an amide-bond formation (2011)
  • Author(s): S. Abe, K. Nakayama, H. Kobayashi, T. Kiba, T. Akasaka, S. Sato, M. Uo, F. Watari, and T. Takada
  • Journal Title: Nano Biomedicine Volume: 3 Pages: 208-216
  • Peer Reviewed
• [Journal Article] Evaporation Processes of Water Molecules from Graphene Edge : DFT and MD Study (2011)
  • Author(s): S. Abe, Y. Nagoya, F. Watari, and H. Tachikawa
  • Journal Title: Computational Material Science Volume: 50 Pages: 2640-2643
  • Peer Reviewed
• [Journal Article] The interaction between carbon nanomaterials and polypeptide : An in vitro and in silico study (2011)
  • Author(s): S. Abe, R. Komine, Y. Nagoya, F. Watari, and H. Tachikawa
  • Journal Title: Molecular Crystals and Liquid Crystals Volume: 538 Pages: 258-264
  • Peer Reviewed
• [Journal Article] Development of a novel transparent substrate coated by carbon nanotubes through covalent bonding (2011)
  • Author(s): S. Abe, K. Nakayama, D. Hayashi, T. Akasaka, M. Uo, F. Watari and T. Takada
  • Journal Title: Physics Procedia Volume: 14 Pages: 147-151
  • Peer Reviewed
• [Journal Article] Observation of 3D network nano-structure of Carbon Nanotubes Scaffold for cultivation (2011)
  • Author(s): S.Abe, K.Ishikawa, A.Hyono, H.Kobayashi, T.Kiba, T.Akasaka, M.Uo, Y.Yawaka, S.Sato, T.Yonezwa, F.Watari
  • Journal Title: J.Surface Science and Nanotechnology Volume: 9 Pages: 80-84
  • Peer Reviewed
• [Journal Article] Density Functional Theory (DFT) Study on the water clusters on Graphene Chip (2011)
  • Author(s): S.Abe, Y.Nagoya, F.Watari, H.Tachikawa
  • Journal Title: Japanese J.Applied Physics Volume: 50 Pages: 01BJ021-23
  • Peer Reviewed
• [Journal Article] Biodistribution and Biocompatibility of poly(lactic acid)-coated SiO2 particles (2011)
  • Author(s): S.Abe, A.Sasaki, N.Iwadera, T.Akasaka, M.Uo, F.Watari
  • Journal Title: Nano Biomedicine Volume: 3 Pages: 300-305
  • Peer Reviewed
• [Journal Article] DFT study on the interaction of Fullerene (C60) with hydroxyl radical (OH) (2011)
  • Author(s): H.Tachikawa, T.Iyama, S.Abe
  • Journal Title: Physics Procedia Volume: 14 Pages: 139-142
  • Peer Reviewed
• [Presentation] SEM observation of carbon nano materials using ionic liquids (2012)
  • Author(s): S.Abe, A.Hyono, T.Yonezawa, F.Watari
  • Organizer: ISPlasma2012
  • Place of Presentation: Chubu University (Kasugai)
  • Year and Date: 2012-03-07
• [Presentation] ナノカーボン物質と生体関連物質との相互作用 (2012)
  • Author(s): 阿部、石川、小峰、亘理、田地川
  • Organizer: 化学系学協会北海道支部2012年冬季研究発表会
  • Place of Presentation: 北海道大学(札幌)
  • Year and Date: 2012-02-01
• [Presentation] Biodistribution and cytocompatibility of micro/nano-sized ceramics particles : an in vivo and in vitro investigation (2011)
  • Author(s): S.Abe, N.Iwadera, T.Akasaka M.Uo, Y.Yawaka, Y.Kuboki, T.Yonezawa, F.Watari
  • Organizer: 11th Asian BioCeramics Symposium
  • Place of Presentation: NIMS (Ibaraki)
  • Year and Date: 2011-12-01
• [Presentation] Internal diffusion of biocompatible polymer-coated inorganic nanoparticles in mice (2011)
  • Author(s): S.Abe, A.Sasaki, T.Narushima Y.Uchida, T.Akasaka, M.Uo, T.Yonezawa, F.Watari
  • Organizer: 23rd International Symposium on Ceramics in Medicine
  • Place of Presentation: Ceylan InterContinental Istanbul Hotel (Turkey)
  • Year and Date: 2011-11-07
• [Presentation] イオン液体を用いたSEMの新規観察法の開発 (2011)
  • Author(s): 阿部、兵野、赤坂、亘理、米澤
  • Organizer: 第58回日本歯科理工学会学術講演会
  • Place of Presentation: 奥羽大学(郡山)
  • Year and Date: 2011-10-23
• [Presentation] Biocompatibility and biodistribution of poly (lactic acid)-coated micro/nano particles (2011)
  • Author(s): S.Abe, A.Sasaki, N.Iwadera, T.Akasaka, M.Uo, F.Watari
  • Organizer: International Union of Materials Research Society-Internatonal Conference in Asia 2011
  • Place of Presentation: Taipei World Trade Center Nangang Exhibition Hall (Chinese Taipei)
  • Year and Date: 2011-09-19
• [Presentation] Morphlogy and photophysical properties of one-dimensional arrayed porphyrin aggregates assisted by cyclodextrin inclusion complexation (2011)
  • Author(s): S.Abe, H.Kobayashi, T.Kiba, F.Watari, S.Sato
  • Organizer: KJF2011
  • Place of Presentation: Hotel Hyundai Gyeongju (South Korea)
  • Year and Date: 2011-09-16
• [Presentation] Morphology size distribution and elemental analysis of several dental working debris (2011)
  • Author(s): S.Abe, N.Iwadera, M.Esaki, K.Aoyama, T.Akasaka, M.Uo, M.Morita, Y.Yawaka, F.Watari
  • Organizer: 3rd International Conference on Surface and Interface of Biomaterials
  • Place of Presentation: Hokkaido University (Sapporo)
  • Year and Date: 2011-07-14
• [Presentation] Biodistribution of micro-/nano-sized particles and their cytocoxisity (2011)
  • Author(s): S.Abe, N.Iwadera, K.Ishikawa, S.Itoh, T.Akasaka, M.Uo, Y.Yawaka, Y.Kuboki, T.Yonezawa, F.Watari
  • Organizer: International Dental Materials Congress 2011
  • Place of Presentation: Yonsei University (South Korea)
  • Year and Date: 2011-05-28
• [Presentation] イオン液体を用いた細胞培養用カーボンナノチューブスキャフォルドの表面構造観察 (2011)
  • Author(s): 阿部、兵野、亘理、米澤
  • Organizer: 日本顕微鏡学会第67回学術講演会
  • Place of Presentation: 福岡国際会議場(福岡)
  • Year and Date: 2011-05-16
• [Book] 『ナノ材料のリスク評価と安全性対策』第4章4「XSAMによるナノ粒子体内動態の可視化」 (2010)
  • Author(s): 亘理文夫(編集)、阿部薫明(分担執筆)
  • Total Pages: 317
  • Publisher: フロンティア出版
• [Remarks]
  • URL: http://abeshy.web.fc2.com/