2010 Fiscal Year Final Research Report

Induction of antimicrobial proteins by colloid solution of platinum nanoparticles.

Research Project

Project/Area Number	21791946
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Dental engineering/Regenerative dentistry
Research Institution	Health Sciences University of Hokkaido
Principal Investigator	NAGANO Futami Health Sciences University of Hokkaido, 歯学部, 助教 (10534448)
Project Period (FY)	2009 – 2010
Keywords	生体材料学
Research Abstract	The aim of this study was to evaluate the induction of antimicrobial protein, such as S100A7, S100A15 and Chromogranin A by stimulation of colloid solution of platinum nanoparticles (CPN) on human gingival fibroblast, pulp fibroblast and KB cell. We proved that CPN induced the expression of antimicrobial protein. Also we found that the induction of gene expression of all the candidate genes was necessarily bound to the induction of the MAP-Kinase pathway like ERK 1/2, p-38 and JNK as proved by Immunoblotting experiments. ASK involvement as well as the phosphorylation of ASK has been evidenced in our experiments.