2010 Fiscal Year Final Research Report

The development of cell spheroid implantation therapy using the peripheral blood and the closed culture system

Research Project

Project/Area Number	21791974
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Surgical dentistry
Research Institution	The University of Tokyo
Principal Investigator	SUENAGA Hideyuki The University of Tokyo, 医学部附属病院, 助教 (10396731)
Project Period (FY)	2009 – 2010
Keywords	移植・再生医療 / 発生・分化 / 三次元培養 / 細胞塊
Research Abstract	It is well known that mesenchymal cell condensation plays an important role in the early stages of membranous osteogenesis, three-dimensional and dynamic flow can positively enhance the osteodifferentiation. In this study, we tried to conduct bone tissue engineering without any synthetic or exogenous biomaterials. To imitate bone development, we generated three-dimensional cellular spheroid using bone marrow derived stromal cells and peripheral blood mononuclear cells by rotation culture, then implanted them into the nude rat calvarial defects. We evaluated the bone regenerative capacity of the rats by histological, immunochemical, maicro CT, raman spectrum analysis and mechanical analysis.

(4 results)