Analysis of candidate genes for cleft lip and palate

2010 Fiscal Year Final Research Report

• Project/Area Number: 21792014
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Kyushu University
• Principal Investigator: ARAI Shinsaku Kyushu University, 大学病院, 医員 (40529806)
• Project Period (FY): 2009 – 2010
• Keywords: 口唇口蓋裂 / 関連解析 / SNP

Research Abstract

Cleft palate is one of the most common craniofacial deformities. Because Sprouty2 has recently been reported to play a key role in the occurrence of cleft palate in humans, we researched the role of Sprouty2 in mice palatogenesis. Sprouty2-deficient palates fused completely in palatal organ culture. However, palate mesenchymal cell proliferation estimated by Ki-67 staining was increased in Sprouty2 KO mice compared with WT mice. Sprouty2-null palates expressed higher levels of FGF target genes, such as Msx1, Etv5, and Ptx1 than WT controls. Furthermore, proliferation and the extracellular signal-regulated kinase (Erk) activation in response to FGF was enhanced in palate mesenchymal cells transfected with Sprouty2 small interfering RNA. These results suggest that Sprouty2 regulates palate mesenchymal cell proliferation via FGF signaling and is involved in palatal shelf elevation.

Research Products

• [Journal Article] Sprouty2 controls proliferation of palate mesenchymal cells via fibroblast growth factor signaling (2011)
  • Author(s): Kaori MATSUMURA, Takaharu TAKETOMI, Keigo YOSHIZAKI, Shinsaku ARAI, Terukazu SANUI, Daigo YOSHIGA, Akihiko YOSHIMURA, Seiji NAKAMURA
  • Journal Title: Biochemical and Biophysical Research Communications 404 Pages: 1076-1082
• [Journal Article] 異常構音を有する口蓋裂患者の語音弁別能に関する検討 (2010)
  • Author(s): 中間友美、笹栗正明、松崎幸代、緒方祐子、光安岳志、新井伸作、松村香織、中村誠司
  • Journal Title: 日本口蓋裂学会雑誌 35(3) Pages: 186-194
• [Presentation] MAPK抑制因子Sprouty2のFGFシグナルを介した口蓋間葉細胞増殖制御 (2010)
  • Author(s): 松村香織、武富孝治、笹栗正明、光安岳志、吉賀大午、新井伸作、前原隆、讃井彰一、中村誠司
  • Organizer: 第55回日本口腔外科学会総会・学術大会
  • Place of Presentation: 千葉
  • Year and Date: 20101000
• [Presentation] Sprouty2 deficient mice exhibit cleft palate. (2010)
  • Author(s): Kaori MATSUMURA, Takaharu TAKETOMI, Daigo YOSHIGA, Shinsaku ARAI, Takashi MAEHARA, Terukazu SANUI, Kazuaki NONAKA, Akihiko YOSHIMURA, Seiji NAKAMURA
  • Organizer: International Association of DentalResearch Barcelona
  • Place of Presentation: Spain
  • Year and Date: 20100700
• [Presentation] Evaluation of re-pushback surgery for cleft palate patients with severe velopharyngeal incompetence (2010)
  • Author(s): Shinsaku ARAI, Masaaki SASAGURI, Takeshi MITSUYASU, Tomomi NAKAMA, Kaori MATSUMURA, Seiji NAKAMURA
  • Organizer: 6th Congress of the International Cleft Lip and Palate Foundation Seoul
  • Place of Presentation: Korea
  • Year and Date: 20100600
• [Presentation] Sprouty2 deficient mice exhibit cleft palate. (2010)
  • Author(s): Kaori MATSUMURA, Takaharu TAKETOMI, Masaaki SASAGURI, Takeshi MITSUYASU, Tomomi TSUJIGUCHI, Shinsaku ARAI, Daigo YOSHIGA, Takashi MAEHARA, Terukazu SANUI, Akihiko YOSHIMURA, Seiji NAKAMURA
  • Organizer: 6th Congress of the International Cleft Lip and Palate Foundation Seoul
  • Place of Presentation: Korea
  • Year and Date: 20100600
• [Presentation] A study of speech discrimination ability of cleft palate patients with abnormal articulation (2010)
  • Author(s): Tomomi NAKAMA, Masaaki SASAGURI, Takeshi MITSUYASU, Shinasku ARAI, Kaori MATSUMURA, Seiji NAKAMURA
  • Organizer: 6th Congress of the International Cleft Lip and Palate Foundation Seoul
  • Place of Presentation: Korea
  • Year and Date: 20100600
• [Presentation] Sprouty2はマウス口蓋癒合に関与する (2010)
  • Author(s): 松村香織、武富孝治、笹栗正明、光安岳志、吉賀大午、新井伸作、前原隆、讃井彰一、中村誠司
  • Organizer: 第64回日本口腔科学会総会・学術大会
  • Place of Presentation: 北海道
  • Year and Date: 20100600
• [Presentation] マウス口蓋形成におけるSprouty2の役割 (2010)
  • Author(s): 松村香織、武富孝治、笹栗正明、光安岳志、辻口友美、吉賀大午、新井伸作、吉崎恵悟、中村誠司
  • Organizer: 第34回日本口蓋裂学会総会・学術大会
  • Place of Presentation: 東京
  • Year and Date: 20100500
• [Presentation] 摂食・嚥下障害を認めた重度多発異常を伴口蓋裂症例に対する 口蓋形成術の意義 (2010)
  • Author(s): 光安岳志、笹栗正明、金城亜紀、大部一成、辻口友美、新井伸作、松村香織、中村誠司
  • Organizer: 第34回日本口蓋裂学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 20100500
• [Presentation] 片側性不完全口唇裂症例における術前外鼻矯正の有用性の検討 (2009)
  • Author(s): 光安岳志、笹栗正明、中間友美、松村香織、新井伸作、中村誠司
  • Organizer: 第54回日本口腔外科学会総会・学術大会
  • Place of Presentation: 北海道
  • Year and Date: 20091000
• [Presentation] 異常構音を有する口蓋裂患者の語音弁別に関する検討 (2009)
  • Author(s): 中間友美、笹栗正明、松崎幸代、緒方祐子、光安岳志、新井伸作、松村香織、中村誠司
  • Organizer: 第33回日本口蓋裂学会総会
  • Place of Presentation: 東京
  • Year and Date: 20090500
• [Remarks] ホームページ等