BRAK is a new type marker for development of tailor made medicine in head and neck squamous cell carcinoma.

2010 Fiscal Year Final Research Report

• Project/Area Number: 21792037
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Kanagawa Dental College
• Principal Investigator: OZAWA Shigeyuki Kanagawa Dental College, 歯学部, 助教 (40434394)
• Project Period (FY): 2009 – 2010
• Keywords: ケモカイン / BRAK / 扁平上皮癌

Research Abstract

BRAK is not expressed or negligible level in head and neck squamous cell carcinoma. We cleared that methylation of BRAK promoter regulates the expression of BRAK as a negative regulator.

Research Products

• [Journal Article] Reactive oxygen species (ROS) reduce the expression of BRAK/CXCL14 in human head and neck squamous cell carcinoma cells. (2010)
  • Author(s): Maehata Y, Ozawa S, Kobayashi K, Kato Y, Yoshino F, Miyamoto C, Izukuri K, Kubota E, Hata R, Lee MC.
  • Journal Title: Free Radio Res 44(8) Pages: 913-924
  • Peer Reviewed
• [Journal Article] Chemokine CXCL14/BRAK transgenic mice suppress growth of carcinoma cell transplants. (2010)
  • Author(s): Izukuri K, Suzuki K, Yajima N, Ozawa S, Ito S, Kubota E, Hata RI.
  • Journal Title: Transgenic Res
  • Peer Reviewed
• [Journal Article] Expression of a chemokine BRAK/CXCL14 in oral floor carcinoma cells reduces the settlement rate of the cells and suppresses their proliferation in vivo. (2010)
  • Author(s): Ito S, Ozawa S, Ikoma T, Yajima N, Kiyono T, Hata R.
  • Journal Title: Biomed Res. 31(3) Pages: 199-206
  • Peer Reviewed
• [Journal Article] Human p38 delta MAP kinase mediates UV irradiation induced up-regulation of the gene expression of chemokine BRAK/CXCL14. (2010)
  • Author(s): Ozawa S, Ito S, Kato Y, Kubota E, Hata R.
  • Journal Title: Biochem Biophys Res Commun. 396(4) Pages: 1060-1064
  • Peer Reviewed
• [Journal Article] Functional characterization of proximal promoter of gene for human BRAK/CXCL14, a tumor-suppressing chemokine. (2010)
  • Author(s): Komori R, Ozawa S, Kato Y, Shinji H, Kimoto S, Hata R.
  • Journal Title: Biomed Res. 31(2) Pages: 123-131
  • Peer Reviewed
• [Journal Article] Expression of tumour-suppressing chemokine BRAK/CXCL14 reduces cell migration rate of HSC-3 tongue carcinoma cells and stimulates attachment to collagen and formation of elongated focal adhesions in vitro. (2010)
  • Author(s): Sato K, Ozawa S, Izukuri K, Kato Y, Hata R.
  • Journal Title: Cell Biol Int. 34(5) Pages: 513-522
  • Peer Reviewed
• [Journal Article] BRAK/CXCL14 expression in oral carcinoma cells completely suppresses tumor cell xenografts in SCID mouse. (2009)
  • Author(s): Ozawa S, Kato Y, Kubota E, Hata R.
  • Journal Title: Biomed Res. 30(5) Pages: 315-318
  • Peer Reviewed
• [Journal Article] Restoration of BRAK / CXCL14 gene expression by gefitinib is associated with antitumor efficacy of the drug in head and neck squamous cell carcinoma. (2009)
  • Author(s): Ozawa S, Kato Y, Ito S, Komori R, Shiiki N, Tsukinoki K, Ozono S, Maehata Y, Taguchi T, Imagawa-Ishiguro Y, Tsukuda M, Kubota E, Hata R.
  • Journal Title: Cancer Sol. 100(11) Pages: 2202-2209
  • Peer Reviewed
• [Presentation] Oxidative stress reduce expression of CXCL14/BRAK in human cells (2011)
  • Author(s): MAEHATA Y, OZAWA S, MIYAMOTO C, KOBAYASHI K, KOMORI R, HATA R.-I, LEE M.C
  • Organizer: IADR General Session
  • Place of Presentation: San Diego
  • Year and Date: 20110316-20110319
• [Presentation] Analysis of promoter region of huma BRAK/CXCL14 in SCC cells. (2011)
  • Author(s): KOMORI R, OZAWA S, KATO Y, MAEHATA Y, SHINJI H, HATA R.-I, KIMOTO S
  • Organizer: IADR General Session
  • Place of Presentation: San Diego
  • Year and Date: 20110316-20110319
• [Presentation] Gene expression signatures at acidic extracellular pH in metastatic melanoma cells. (2010)
  • Author(s): Kato Y, Ozawa S, Maehata Y, Maeda T, Kubota E, Tsukuda M, Miyazaki K, Hata R
  • Organizer: Joint MRS-AACR Conference. Metastasis and the Tumor Microenvironment
  • Place of Presentation: Philadelphia
  • Year and Date: 20100912-20100915
• [Presentation] Chemokine CXCL14/BRAK transgenic mice suppress growth and metastasis of carcinoma cells. (2010)
  • Author(s): Hata R, Izukuri K, Suzuki K, Kato Y, Yajima N, Ozawa S, Ito S, Kubota E
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe
  • Year and Date: 2010-08-23
• [Presentation] Chemokine BRAK/CXCL14 is a multifunctional tumor suppressor. (2010)
  • Author(s): Hata R, Izukuri K, Suzuki K, Yajima N, Ozawa S, Ito S, Kubota E
  • Organizer: 88^<th> General Session & Exhibition of the IADR・5^<th> General Session of the Pan European Region of the IADR
  • Place of Presentation: Barcelona
  • Year and Date: 2010-07-14
• [Presentation] BRAK/CXCL14 transgenic mice suppress growth of tumor cell xenografts. (2009)
  • Author(s): Ryu-Ichiro Hata, Kazuhito Izukuri, Kenji Suzuki, Nobuyuki Yajima, Shin Ito, Shigeyuki Ozawa, Eiro Kubota
  • Place of Presentation: Boston
  • Year and Date: 20091115-20091119
• [Presentation] Expression of BRAK/CXCL14 is associated with antitumor efficacy of gefitinib in head and neck squamous cell carcinoma (2009)
  • Author(s): Ryu-Ichiro Hata, Shigeyuki Ozawa, Yasumasa Kato, Shin Ito, Reika Komori, Naoto Shiiki, Keiichi Tsukinoki, Yojiro Maehata, Eiro Kubota
  • Organizer: ECCO15 and 34^<th> ESMO Multidisciplinary Congress
  • Place of Presentation: Berlin
  • Year and Date: 20090920-20090924
• [Presentation] Functional Analysis of Promoter of Human BRAK/CXCL14, A Tumor Suppressor. (2009)
  • Author(s): Reika Komori, Shigeyuki Ozawa, Yasumasa Kato, Hisaaki Shinji, Shigenari Kimoto, Ryu-Ichiro Hata
  • Organizer: 87^<th> General Session & Exhibition of the IADR・38^<th> Annual Meeting of the AADR・33^<rd> Annual Meeting of the CADR
  • Place of Presentation: Miami Beach Convention Center, Miami, Florida
  • Year and Date: 20090401-20090404
• [Presentation] A new mechanism of tumor suppression by gefitinib in HNSCC. (2009)
  • Author(s): Shigeyuki Ozawa, Yasumasa Kato, Shin Ito, Reika Komori, Naoto Shiiki, Keiichi Tukinoki, Satoru Ozono, Yojiro Maehata, Takahide Taguchi, Yukari Imagawa-Ishiguro, Mamoru Tsukuda, Eiro Kubota, Ryu-Ichiro Hata
  • Organizer: 87^<th> General Session & Exhibition of the IADR・38^<th> Annual Meeting of the AADR・33^<rd> Annual Meeting of the CADR
  • Place of Presentation: Miami Beach Convention Center, Miami, Florida
  • Year and Date: 20090401-20090404
• [Presentation] Suppression of growth of Lewis lung carcinoma cell xenografts in BRAK transgenic mouse (2009)
  • Author(s): Kazuhito Izukuri, Kenji Suzuki, Shigeyuki Ozawa, Eiro Kubota, Ryu-Ichiro Hata
  • Organizer: Production of cancer resistant mouse. Yokosuka Science Festa
  • Place of Presentation: Shonan Village Center, Hayama
  • Year and Date: 2009-07-06
• [Presentation] Chemokine BRAK stimulates apoptosis elicited by gefitinib in oral squamous cell carcinoma (2009)
  • Author(s): Shin Ito, Shigeyuki Ozawa, Naoto Shiiki, Keiichi Tsukinoki, Eiro Kubota, Yasumasa Kato, Takahide Taguchi, Yukari Imagawa-Ishiguro, Mamoru Tsukuda, Ryu-Ichiro Hata
  • Organizer: Yokosuka Science Festa
  • Place of Presentation: Shonan Village Center, Hayama
  • Year and Date: 2009-07-06
• [Presentation] Expression of BRAK/CXCL14 is associated with antitumor efficacy of gefitinib in head and neck squamous cell carcinoma. (2009)
  • Author(s): Shigeyuki Ozawa, Yasumasa Kato, Shin Ito, Reika Komori, Kenji Suzuki, Keiichi Tsukinoki, Yojiro Maehata, Takahide Taguchi, Yukari Imagawa-Ishiguro, Mamoru Tsukuda, Eiro Kubota, Ryu-Ichiro Hata
  • Organizer: Yokosuka Science Festa
  • Place of Presentation: Shonan Village Center, Hayama
  • Year and Date: 2009-07-06
• [Presentation] Functional analysis of promoter region of humanBRAK/CXCL14 (2009)
  • Author(s): Reika Komori, Shigeyuki Ozawa, Yasumasa Kato, Hisaaki Shinji, Shigenari Kimoto, Ryu-Ichiro Hata
  • Organizer: a tumor progression suppressor. Yokosuka Science Festa
  • Place of Presentation: Shonan Village Center, Hayama
  • Year and Date: 2009-07-06
• [Presentation] Reactive oxygen species reduce the expression of BRAK/CXCL14 in human head and neck squamous cell carcinoma cells. (2009)
  • Author(s): Yojiro Maehata, Shigeyuki Ozawa, Chihiro Miyamoto, Kyo Kobayashi, Yasumasa Kato, Fumihiko Yoshino, Masaichi-Chang-il Lee, Ryu-Ichirc Hata
  • Organizer: Yokosuka Science Festa
  • Place of Presentation: Shonan Village Center, Hayama
  • Year and Date: 2009-07-05