2010 Fiscal Year Final Research Report

The elucidation of function of SIRT1on bone and periodontal tissue

Research Project

Project/Area Number	21792130
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Periodontal dentistry
Research Institution	Showa University
Principal Investigator	TAKADA Takatora Showa University, 歯学部, 普通研究生 (20384323)
Project Period (FY)	2009 – 2010
Keywords	SIRT1 / 骨代謝 / 歯周組織
Research Abstract	Sirt1 is known as regulator of the aging process. We examined a function of Sirt1 in gingival epithelial cells and osteoclasts which constituted periodontium. In a gingival epithelia cell, LPS increased the levels of Sirt1. We examined the role of Sirt1 for the production of TNF-α by LPS in gingival epithelial cells. Although LPS increased TNF-α and NF-kB activation, sirtinol, sirt1 inhibitor prevented LPS-induced TNF-α. On the other hand, we treated resveratrol, Sirt1 activator in osteoclast formation assay to elucidate a function of Sirt1 in osteoclatogenesis. Resveratrol increased the number of RANKL-induced osteoclasts. We investigated osteoclast-related gene expression using microarray system to clarify whether activation of Sirt1 controlled the osteoclast formation. The levels of cathepsin K and NFATc1 gene expression were significantly decreased.