2010 Fiscal Year Final Research Report
Development of methods to prevent alveolar bone absorption during periodontitis using epigenetic regulation
| Project/Area Number |
21792160
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Social dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
TSUDA Hiromasa Nihon University, 歯学部, 助教 (60325470)
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| Project Period (FY) |
2009 – 2010
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| Keywords | ヒストンアセチル化 / ヒストンメチル化 / 細胞死 / 破骨細胞 / 予防法 |
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| Research Abstract |
In this study, we examined a hypothesis that modulation of histone acetylation or methylation may also have some effect on the osteoclast differentiation. RANKL treatment induced the number of multinucleated osteoclast-like cells and the TRAP activities. Both histone deacetylase and methyltransferase inhibiton decreased the number of RANKL-induced osteoclast-like cells and TRAP activities in a dose-dependent manner. During the experiments, we also observed that RANKL treatment induced Raw264.7 cell death and the histone-deacetylase or histone methyltransferase-inhibitor dose-dependently suppressed the RANKL-induced cell death.
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