2010 Fiscal Year Final Research Report

Development of methods to prevent alveolar bone absorption during periodontitis using epigenetic regulation

Research Project

Project/Area Number	21792160
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Social dentistry
Research Institution	Nihon University
Principal Investigator	TSUDA Hiromasa Nihon University, 歯学部, 助教 (60325470)
Project Period (FY)	2009 – 2010
Keywords	ヒストンアセチル化 / ヒストンメチル化 / 細胞死 / 破骨細胞 / 予防法
Research Abstract	In this study, we examined a hypothesis that modulation of histone acetylation or methylation may also have some effect on the osteoclast differentiation. RANKL treatment induced the number of multinucleated osteoclast-like cells and the TRAP activities. Both histone deacetylase and methyltransferase inhibiton decreased the number of RANKL-induced osteoclast-like cells and TRAP activities in a dose-dependent manner. During the experiments, we also observed that RANKL treatment induced Raw264.7 cell death and the histone-deacetylase or histone methyltransferase-inhibitor dose-dependently suppressed the RANKL-induced cell death.

(2 results)