2010 Fiscal Year Final Research Report

Constructive research for understanding the effects of amyloid β to neuronal function.

Research Project

Project/Area Number	21800016
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Neurochemistry/Neuropharmacology
Research Institution	Tokyo University of Technology (2010) Tokyo Medical and Dental University (2009)
Principal Investigator	SUZUKI Ikuro Tokyo University of Technology, 応用生物学部, 助教 (90516311)
Project Period (FY)	2009 – 2010
Keywords	神経細胞 / アミロイドβ / 多電極アレイ / 時系列解析 / チモキノン / 長期計測 / DFA / アルツハイマー病
Research Abstract	Alzheimer's disease (AD) is characterized by the aggregation of amyloid β peptide (Aβ). The aim of this study is to investigate the effect of Amyloid Beta toxicity in embryonic rat hippocampus through chronic monitoring of neuronal cell electrical activity using multielectrode array (MEA) recordings, and the molecules protecting neurons from Aβ toxicity. Applying Aβ to cultured embryonic rat hippocampal neurons has an inhibitory effect on spontaneous activity started 24h after applying and the firing disappeared at 72h. When thymoquinone (TQ) active constituent of Nigella Sativa Oil and Aβ were simultaneously applied, thymoquinone reduces the deleterious effect of Aβ at 34h after applying. This results indicated that TQ have the potential protecting hippocampus cells from Aβ toxicity.

(6 results)