2010 Fiscal Year Final Research Report
In vivo chronic imaging of neuronal circuit plasticity using a genetically-encoded calcium sensor
| Project/Area Number |
21800091
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
SATO Masaaki The Institute of Physical and Chemical Research, シナプス機能研究チーム, 研究員 (90518325)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 蛍光カルシウムバイオセンサー / ウイルスベクター / トランスジェニックマウス / 子宮内電気穿孔法 / 二光子レーザー顕微鏡 / 記憶・学習 / 可塑性 |
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| Research Abstract |
To establish chronic in vivo two-photon calcium imaging, we generated transgenic mice that stably express a genetically-encoded calcium sensor GCaMP4 in layer 5 of cortex and CA1 of hippocampus. Our in vivo two-photon imaging confirmed that the fluorescence of GCaMP4 in hippocampal CA1 pyramidal cells of these mice increased rapidly and substantially in response to pharmacologically-induced elevation of neuronal circuit activity. These transgenic mice will thus become a valuable tool for longitudinal imaging studies of learning-induced neuronal circuit plasticity in the brain.
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Research Products
(3 results)
