2010 Fiscal Year Final Research Report
Construction of Protein-RNA conjugate for intracellular imaging of metabolites
| Project/Area Number |
21850026
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemistry related to living body
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| Research Institution | Konan University |
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Principal Investigator |
ENDOH Tamaki Konan University, 先端生命工学研究所, 講師 (90550236)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 代謝産物検 / 細胞内イメージング / RNA-タンパク質相互作用 / アロステリック相互作用 / RNA構造変化 |
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| Research Abstract |
In this study, construction of Protein-RNA conjugate, which enables detection of small molecules, was performed. RNA libraries that consist of artificial RNA aptamer and TAR-RNA were designed. RNAs that allosterically interact with Tat-peptide in the presence of the target small molecules were selected from the libraries. By cooperative function of artificially modified Luciferase containing Tat-peptide, the selected RNAs enabled luminometric detection of the small molecules. Additionally, direct conjugation of RNA aptamer and TAR-RNA enabled detection of small molecule by an intracellular gene expression though an allosteric interaction of Tat protein.
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