• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2010 Fiscal Year Final Research Report

Quantitative analysis of protein-protein interaction inside animal-derived cells

Research Project

  • PDF
Project/Area Number 21850027
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Chemistry related to living body
Research InstitutionKonan University

Principal Investigator

USUI Kenji  Konan University, フロンティアサイエンス学部, 講師 (70543792)

Project Period (FY) 2009 – 2010
Keywordsタンパク質 / 発現制御 / 細胞アレイ / ナノバイオ / タンパク質の自己相互作用 / ペプチド核酸(PNA) / 核酸の四重鎖構造 / アミロイド
Research Abstract

Construction of quantitative analysis system for protein-protein interaction inside animal-derived cells was attempted with controlling those protein expression levels using peptide nucleic acid (PNA)-peptide conjugates. We successfully achieved three indispensable basic elements for the system as follows: (1) construction of novel protein-expression control system, (2) construction of detection system for self-assembly reaction as a first step in establishing the analysis system of protein-protein interaction inside cells, (3) construction of cell array system for high-throughput analysis.

  • Research Products

    (30 results)

All 2011 2010 2009 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (16 results) Book (3 results) Remarks (3 results)

  • [Journal Article] Thermodynamic stability of Hoogsteen and Watson-Crick base pairs in the presence of histone H3-mimicking peptide2011

    • Author(s)
      S.Pramanik, K.Nakamura, K.Usui, S.Nakano, S.Saxena, J.Matsui, D.Miyoshi, N.Sugimoto
    • Journal Title

      Chem.Commun. 47

      Pages: 2790-2792

    • Peer Reviewed
  • [Journal Article] Nanomolar aggregation of amyloid-beta peptides covalently and site-specifically modified by cholesterol oxidation products2010

    • Author(s)
      K.Usui, J.D.Hulleman, J.F.Paulsson, S.J.Siegel, E.T.Powers, J.W.Kelly
    • Journal Title

      Peptides, 2008 2008

      Pages: 588-599

    • Peer Reviewed
  • [Journal Article] Construction of detection system for Amyloid beta peptide localization and aggregation using fluorescent and luminescent fusion proteins2010

    • Author(s)
      K.Usui, M.Mie, T.Andou, N.Sugimoto, H.Mihara, E.Kobatake
    • Journal Title

      Peptide Science 2009 (K.Okamoto, Ed.) 2009

      Pages: 111-112

    • Peer Reviewed
  • [Journal Article] Fluorescent and luminescent fusion proteins for detection of Amyloid beta peptide localization and aggregation2010

    • Author(s)
      K.Usui, M.Mie, T.Andou, N.Sugimoto, H.Mihara, E.Kobatake
    • Journal Title

      Peptides 2010 2010

      Pages: 488-489

    • Peer Reviewed
  • [Journal Article] PNA-peptide conjugates for regulation of DNA and RNA G-quadruplex structures depending on a particular protease concentration2010

    • Author(s)
      K.Usui, K.Kobayashi, N.Sugimoto
    • Journal Title

      Peptides 2010 2010

      Pages: 608-609

    • Peer Reviewed
  • [Journal Article] Protein-protein interactions and selection : array-based techniques for screening disease-associated biomarkers in predictive/early diagnosis2010

    • Author(s)
      K.-Y. Tomizaki, K.Usui, H.Mihara
    • Journal Title

      FEBS J. 277

      Pages: 1996-2005

    • Peer Reviewed
  • [Journal Article] A designed peptide chip : protein fingerprinting technology with a dry peptide array and statistical data mining2009

    • Author(s)
      K.Usui, K.-Y. Tomizaki, H.Mihara
    • Journal Title

      Methods.Mol.Biol. 570

      Pages: 273-284

    • Peer Reviewed
  • [Journal Article] Site-specific modification of Alzheimer's peptides by cholesterol oxidation products enhances aggregation energetics and neurotoxicity2009

    • Author(s)
      K.Usui, J.D.Hulleman, J.F.Paulsson, S.J.Siegel, E.T.Powers, J.W.Kelly
    • Journal Title

      Proc.Natl.Acad.Sci.USA 106

      Pages: 18563-18568

    • Peer Reviewed
  • [Presentation] 蛍光標識ペプチドとジチオカルバメート鉄錯体による新規NOセンサーの構築2011

    • Author(s)
      宮崎洋、臼井健二、藤井敏司
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川県横浜市
    • Year and Date
      20110326-20110329
  • [Presentation] PNA含有ペプチドを用いてDNA四重鎖構造を特定プロテアーゼの有無によって制御する2011

    • Author(s)
      臼井健二、小林慶太、杉本直己
    • Organizer
      第13回生命化学研究会シンポジウム
    • Place of Presentation
      宮城県仙台市
    • Year and Date
      2011-01-07
  • [Presentation] Nanomolar aggregation and neurotoxicity of Amyloid beta peptides with site-specific modification of oxidized cholesterol2010

    • Author(s)
      K.Usui, E.T.Powers, J.F.Paulsson\, S.J.Siegel, J.W.Kelly
    • Organizer
      5th International Peptide Symposium
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20101204-20101209
  • [Presentation] Designed peptide libraries for protein and cell analyses2010

    • Author(s)
      T.Kikuchi, K.Usui, T.Takahashi, H.Mihara
    • Organizer
      5th International Peptide Symposium
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20101204-20101209
  • [Presentation] 核酸の四重鎖構造を特定のプロテアーゼの有無によって制御できるPNA含有ペプチド2010

    • Author(s)
      臼井健二、小林慶太、杉本直己
    • Organizer
      第4回バイオ関連化学シンポジウム
    • Place of Presentation
      大阪府豊中市
    • Year and Date
      20100924-20100926
  • [Presentation] Enhancement of aggregation energetics and neurotoxicity by site-specific modification of amyloid beta peptides with oxidized cholesterol2010

    • Author(s)
      K.Usui, E.T.Powers, J.F.Paulsson, S.J.Siegel, J.W.Kelly
    • Organizer
      31st European Peptide Symposium
    • Place of Presentation
      Copenhagen, Denmark
    • Year and Date
      20100905-20100909
  • [Presentation] PNA-peptide conjugates for regulation of DNA and RNA G-quadruplex structures depending on a particular protease concentration2010

    • Author(s)
      K.Usui, K.Kobayashi, N.Sugimoto
    • Organizer
      31st European Peptide Symposium
    • Place of Presentation
      Copenhagen, Denmark
    • Year and Date
      20100905-20100909
  • [Presentation] Fluorescentand luminescent fusion proteins for detection of Amyloid beta peptide localization and aggregation2010

    • Author(s)
      K.Usui, M.Mie, T.Andou, N.Sugimoto, H.Mihara, E.Kobatake
    • Organizer
      31st European Peptide Symposium
    • Place of Presentation
      Copenhagen, Denmark
    • Year and Date
      20100905-20100909
  • [Presentation] Data mining of protein fingerprints and cell fingerprints from designed alpha-helical peptide arrays2010

    • Author(s)
      臼井健二
    • Organizer
      第4回HiPep沖縄国際ワークショップ
    • Place of Presentation
      沖縄県那覇市
    • Year and Date
      20100709-20100711
  • [Presentation] αへリックスペプチドの細胞導入活性スクリーニング及び細胞死活性評価2010

    • Author(s)
      菊池卓哉、臼井健二、高橋剛、三原久和
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      大阪府東大阪市
    • Year and Date
      20100326-20100329
  • [Presentation] 核酸四重鎖構造を制御するPNA含有ペプチドの構築2010

    • Author(s)
      小林慶太、臼井健二、杉本直己
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      大阪府東大阪市
    • Year and Date
      20100326-20100329
  • [Presentation] タンパク質解析・細胞解析用ペプチドチップ2010

    • Author(s)
      臼井健二
    • Organizer
      バイオプロセッシング研究会(バイオ単分子研究会)
    • Place of Presentation
      東京都千代田区
    • Year and Date
      2010-03-23
  • [Presentation] Screening of peptides with cell penetrating activity against various types of cells from a novel alpha-helical peptide library2009

    • Author(s)
      T.Kikuchi, K.Usui, T.Takahashi, H.Mihara
    • Organizer
      3rd Asia-Pacific International Peptide Symposium
    • Place of Presentation
      Jeju (Korea)
    • Year and Date
      20091108-20091111
  • [Presentation] Construction of detection system for Amyloid beta peptide localization and aggregation using fluorescent and luminescent fusion proteins2009

    • Author(s)
      K.Usui, M.Mie, T.Andou, N.Sugimoto, H.Mihara, E.Kobatake
    • Organizer
      第46回ペプチド討論会
    • Place of Presentation
      福岡県北九州市
    • Year and Date
      20091104-20091106
  • [Presentation] 細胞内外でのアミロイドβペプチドの局在と構造変化を検出できる発光・蛍光タンパク質の構築2009

    • Author(s)
      臼井健二、三重正和、杉本直己、三原久和、小畠英理
    • Organizer
      第24回生体機能関連化学シンポジウム,第12回バイオテクノロジー部会シンポジウム
    • Place of Presentation
      福岡県福岡市
    • Year and Date
      20090913-20090915
  • [Presentation] 種々の細胞系を用いた新規αへリックスペプチドの細胞導入活性スクリーニング2009

    • Author(s)
      菊池卓哉、臼井健二、高橋剛、三原久和
    • Organizer
      第24回生体機能関連化学シンポジウム,第12回バイオテクノロジー部会シンポジウム
    • Place of Presentation
      福岡県福岡市
    • Year and Date
      20090903-20090915
  • [Book] 化学フロンティア22 生命現象を理解する分子ツール(第10章 デザインペプチドを用いたプロテインアレイと細胞アレイ)2010

    • Author(s)
      臼井健二,富崎欣也、三原久和
    • Total Pages
      79-87
    • Publisher
      株式会社化学同人
  • [Book] シングルセル解析の最前線(タンパク質・細胞分析用デザインペプチドチップ)2010

    • Author(s)
      臼井健二、菊池卓哉、三原久和
    • Total Pages
      17-24
    • Publisher
      株式会社シーエムシー出版
  • [Book] 超分子サイエンス&テクノロジー(プロテインチップデバイス)2009

    • Author(s)
      臼井健二、富崎欣也、三原久和
    • Total Pages
      1067-1075
    • Publisher
      株式会社エヌ・ティー・エス
  • [Remarks] ホームページ等

    • URL

      http://www.usui-lab.com/

  • [Remarks]

    • URL

      http://www.konan-first.jp/graduate/ti_205_a.html

  • [Remarks]

    • URL

      http://www.konan-first.jp/database/search.php?keyword=&author=2&from_year=0&to_year=0&searchEssay=Search&hideInfo=essay#resultList

URL: 

Published: 2012-01-26   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi