2010 Fiscal Year Final Research Report
Development of a platform for a novel proteome analysis in a living cell utilizing proteoinjection
| Project/Area Number |
21860027
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Biofunction/Bioprocess
|
|---|
| Research Institution | Tokyo University of Agriculture and Technology |
|---|
Principal Investigator |
FUNABASHI Hisakage Tokyo University of Agriculture and Technology, 大学院・工学研究院, 助教 (60552429)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Keywords | バイオテクノロジー / プロテオーム解析 / フェムトインジェクション / 単一細胞解析 / 細胞内機能解析 |
|---|
| Research Abstract |
We have established basic technology for a novel proteome analysis in which the functional rolls of proteins in a living cell are evaluated by the analysis of cellular response induced by the direct introduction of the proteins into a cell (proteoinjection). We have successfully developed a technology which enables us to introduce proteins with functions into a single-target-living ES cell. Moreover, it has been found that the simple insertion of a transcriptional factor specific sequence into a probe DNA enhances the efficiency of the expression of a reporter in a living ES cell. Therefore it has been suggested a new method to evaluate the functional rolls of transcriptional factors inside living ES cells depending on the enhancement of the probe expression efficiency.
|
