2010 Fiscal Year Final Research Report
Development of simple and accurate quantification method of waterborne pathogenic microorganisms using ABC-PCR
| Project/Area Number |
21860094
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Civil and environmental engineering
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| Research Institution | National Institute of Public Health |
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Principal Investigator |
KISHIDA Naohiro 国立保健医療科学院, 生活環境研究部, 主任研究官 (10533359)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 水資源 / 水循環 / 遺伝子 / 病原微生物 / クリプトスポリジウム |
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| Research Abstract |
We describe an assay for simple and accurate quantification of pathogenic microorganisms in water samples using a recently developed quantification method named alternately binding probe competitive PCR(ABC-PCR). Cryptosporidium spp. were selected as representative pathogenic microorganisms in this study. The standard curve of the ABC-PCR assay had a good fitting to a rectangular hyperbola. Concentrations of Cryptosporidium oocysts in real river water samples were successfully quantified by the ABC-PCR assay. The quantified values by the ABC-PCR assay very closely resemble those by the real-time-PCR assay.
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Research Products
(4 results)
