2010 Fiscal Year Final Research Report
Efficient production of novel biosurfactant by yeast isolated from deep sea
| Project/Area Number |
21860097
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Japan Agency for Marine-Earth Science and Technology |
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Principal Investigator |
KONISHI Masaaki Japan Agency for Marine-Earth Science and Technology, 海洋・極限環境生物圏領域, 研究員 (90533860)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 深海 / 酵母 / バイオサーファクタント / マンノシルエリスリトールリピッド / シュードザイマ / シロウリガイ |
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| Research Abstract |
We describe detailed structure determination of biosurfactant produced by Pseudozyma hubeiensis SY62, which was newly isolated from Calyptogena soyoae (deep-sea cold-seep clam, "Shirouri-gai") at 1,156 m in Sagami bay. Results of nuclear magnetic resonance spectroscopies indicated the major product, namely MEL-C, as a promising BS. According to surface tension determination, the novel MEL-C showed larger critical micelle concentration (1.1×10-5 M) than conventional MEL-C which bound C10 and C12 acids (9.1×10-6 M). We demonstrated a highly efficient production of MELs in the improved medium by fed-batch cultivation. The final concentration of MELs reached 129±8.2 g/L for one week.
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