Challenging for finding unknown chlorophyll metabolism enzymes.

2010 Fiscal Year Final Research Report

• Project/Area Number: 21870003
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Hokkaido University
• Principal Investigator: TAKABAYASHI Atsushi Hokkaido University, 低温科学研究所, 助教 (90546417)
• Project Period (FY): 2009 – 2010
• Keywords: クロロフィル代謝 / バイオインフォマティクス

Research Abstract

We have selected the candidates of the 7-hydroxymethyl chlorophyll a reductase based on the bioinformatic method using the co-expression analysis and phylogenetic profiling, and we found the major candidate. In addition, we have comprehensively detected the protein complexes which contain the known enzymes regarding chlorophyll metabolism by BN-PAGE.

Research Products

• [Journal Article] LIL3, a light-harvesting-like protein, plays an essential role in chlorophyll and tocopherol biosynthesis. (2010)
  • Author(s): Ryoichi Tanaka, Maxi Rothbart, Seiko Oka, Atsushi Takabayashi, Kaori Takahashi, Masaru Shibata, Fumiyoshi Myouga, Reiko Motohashi, Kazuo Shinozaki, Bernhard Grimm, Ayumi Tanaka
  • Journal Title: Proc.Natl.Acad.Sci.U S A. 107 Pages: 16721-16725
  • Peer Reviewed
• [Journal Article] ゲノム世代のタンパク質複合体解析 (2010)
  • Author(s): 高林厚史
  • Journal Title: 光合成研究 20 Pages: 60-64
  • Peer Reviewed
• [Journal Article] Three PsbQ-like proteins are required for the function of the chloroplast NAD(P)H dehydrogenase complex in Arabidopsis. (2010)
  • Author(s): Shinya Yabuta, Kentaro Ifuku, Atsushi Takabayashi, Seiko Ishihara, Kunio Ido, Noriko Ishikawa, Tsuyoshi Endo, Fumihiko Sato
  • Journal Title: Plant Cell Physiol. 51 Pages: 866-876
  • Peer Reviewed
• [Journal Article] Direct interaction between KaiA and KaiB revealed by a site-directed spin labeling electron spin resonance analysis. (2010)
  • Author(s): Risa Mutoh, Hiroyuki Mino, Reiko Murakami, Tatsuya Uzumaki, Atsushi Takabayashi, Kentaro Ishii, Masahiro Ishiura
  • Journal Title: Genes to Cells. 15 Pages: 269-280
  • Peer Reviewed
• [Presentation] The application of BN-PAGE for comprehensive detection of the protein complexes in the chloroplast. (2011)
  • Author(s): 高林厚史
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東北大学(仙台市)
  • Year and Date: 2011-03-20
• [Presentation] クロロフィルb蓄積変異株における チラコイド膜タンパク質の網羅的解析 (2010)
  • Author(s): 高林厚史
  • Organizer: 日本植物生理学会
  • Place of Presentation: 熊本大学(熊本市)
  • Year and Date: 2010-03-20