2010 Fiscal Year Final Research Report

Structure Study of β2m Amyloid Fibril using Dynamic Nuclear Polarization-Enhanced High-Sensitivity Solid-State NMR

Research Project

Project/Area Number	21870019
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Structural biochemistry
Research Institution	Osaka University
Principal Investigator	MATSUKI Yoh Osaka University, 蛋白質研究所, 助教 (70551498)
Project Period (FY)	2009 – 2010
Keywords	アミロイド繊維 / 固体NMR / 動的核分極 / 希薄サンプリング
Research Abstract	In this study, (1) more than 200-fold sensitivity enhancement was established for solid-state NMR spectroscopy using Dynamic Nuclear Polarization (DNP). (2) Application of the sparse data sampling technique enabled to detect conventionally unobservable signals. (3) A use of reserve isotope labeling technique resolved many previously unseen signals. (4) A new scheme to detect the molecular interfaces within a fibril was developed for high-selectivity and sensitivity. (5) ss2m was suggested to largely retain its native fold in the fibril, contrary to the widespread belief that amyloid fibril formation generally requires native proteins to adopt a fibril-specific fold.