2010 Fiscal Year Final Research Report
Functional analysis of CD9 molecule and oocyte microvilli in fertilization by novel non-invasive imaging method.
| Project/Area Number |
21870021
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | Osaka University |
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Principal Investigator |
SATOUH Yuhkoh Osaka University, 免疫学フロンティア研究センター, 特任助教 (40545571)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 遺伝子操作動物 / 実験動物 / 受精 / 微絨毛 / 膜融合 |
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| Research Abstract |
In this study, I focus on the function of CD9 molecule and egg microvilli in mammalian fertilization. For that, I set two goals as 1) Analysis of fertilization ability of microvilli less mutant egg, and 2) Visualization of egg microvilli by using novel non-invasive imaging method. As a result until 2011, 1) I have obtained the data which strongly suggesting that the eggs without two of essential genes for constructing microvilli don't have fertilizing ability in vivo. And 2) I have established the imaging system which enable us to visualize egg microvilli in living form. By using the imaging system, I additively succeeded in visualizing the process of sperm-egg fusion in real-time scale for the first time.
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[Journal Article] Most fertilizing mouse spermatozoa begin their acrosome reaction before contact with the zona pellucida during in vitro fertilization.2011
Author(s)
Jin, M., Fujiwara, E., Kakiuchi, Y., Okabe, M., Satouh, Y., et al.
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Journal Title
Proc.Natl.Acad.誌 108(12)巻
Pages: 4892-4896
Peer Reviewed
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[Journal Article] Calsperin is a testis-specific chaperone required for sperm fertility2011
Author(s)
Ikawa, M., Tokuhiro, K., Yamaguchi, R., Benham, A. M., Tamura, T., Wada, I., Satouh, Y., et al.
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Journal Title
Journal of Biological Chemistry誌 286(7)巻
Pages: 5639-5646
Peer Reviewed
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[Journal Article] Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mouse.2010
Author(s)
Fujihara, Y., Murakami, M., Inoue, N., Satouh, Y., et al.
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Journal Title
Journal of Cell Science誌 123巻
Pages: 1531-1536
Peer Reviewed
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