2010 Fiscal Year Final Research Report

Elucidation of the functional mechanism of the novel 2-component K^+/H^+ antiporters found in Escherichia coli and Bacillus subtilis.

Research Project

Project/Area Number	21870035
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Functional biochemistry
Research Institution	Toyo University
Principal Investigator	FUJISAWA Makoto Toyo University, 生命科学部, 助教 (70549641)
Project Period (FY)	2009 – 2010
Keywords	カリウムイオン / アンチポーター / 大腸菌 / 枯草菌 / 分子生物学
Research Abstract	To elucidate the functional mechanism of the novel 2-component K^+/H^+ antiporters composed from a membrane protein and a peripheral protein found in Escherichia coli and Bacillus subtilis, the detection systems of its interaction was established. Peptide tags were introduced into the proteins and the interaction between the proteins from B. subtilis was successfully detected by both pull-down assay and blue-native PAGE.

(2 results)