SUMO mediated cell cycle regulation in Arabidopsis

2010 Fiscal Year Final Research Report

• Project/Area Number: 21870048
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: ISHIDA Takashi The Institute of Physical and Chemical Research, 細胞機能研究ユニット, 基礎科学特別研究員 (00462656)
• Project Period (FY): 2009 – 2010
• Keywords: 細胞周期 / SUMO / 核内倍加 / 翻訳後修飾 / シロイヌナズナ

Research Abstract

I have improved the E. coli expression system for the components of an in vitro SUMOylation assay. Since the expression efficiency of E1 enzymes was not enough, I have modified the vector and culture conditions. The improved system works well.

Research Products

• [Journal Article] Auxin modulates the transition from the mitotic cycle to the endocycle in Arabidopsis (2010)
  • Author(s): Ishida T, Adachi S, Yoshimura M, Shimizu K, Umeda M, Sugimoto K
  • Journal Title: Development Vol.137 Pages: 63-71
  • Peer Reviewed
• [Journal Article] Developmental control of endocycles and cell growth in plants (2010)
  • Author(s): Breuer C, Ishida T, Sugimoto K
  • Journal Title: Current Opinion in Plant Biology Vol.13 Issue 6 Pages: 654-660
  • Peer Reviewed
• [Journal Article] SUMO E3 Ligase HIGH PLOIDY2 Regulates Endocycle Onsetand Meristem Maintenance in Arabidopsis (2009)
  • Author(s): Ishida T, Fujiwara S, Miura K, Stacey N, Yoshimura M, Schneider K, Adachi S, Minamisawa K, Umeda M, Sugimoto K
  • Journal Title: The Plant Cell Vol.21 Pages: 2284-2297
  • Peer Reviewed
• [Journal Article] Functional Analyses of LONELY GUY Cytokinin-Activating Enzymes Reveal the Importance of the Direct Activation Pathway in Arabidopsis (2009)
  • Author(s): Kuroha T, Tokunaga H, Kojima M, Ueda N, Ishida T, Nagawa S, Fukuda H, Sugimoto K, Sakakibara H
  • Journal Title: The Plant Cell Vol.21 Pages: 3152-3169
  • Peer Reviewed
• [Journal Article] 核内倍加-自発的に倍加してしまう染色体の制御 (2009)
  • Author(s): Ishida T, Sugimoto K
  • Journal Title: 生物の科学 遺伝 Vol.63 No.3 Pages: 24-29
• [Presentation] 新規SUMO E3 ligase HIGH PLOIDY 2の分裂組織における機能の解析 (2011)
  • Author(s): 石田喬志,吉村美香,杉本慶子
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台市 日本
  • Year and Date: 2011-03-20
• [Presentation] SUMO E3 Ligase HIGH PLOIDY2 Regulates Endocycle Onset and Meristem Maintenance in Arabidopsis (2010)
  • Author(s): Ishida T., Fujiwara S., Miura K., Stacey N., Yoshimura M., Schneider K., Adachi S., Minamisawa K., Umeda M., Sugimoto K.
  • Organizer: 21st International Conference on Arabidopsis Research
  • Place of Presentation: Yokohama
  • Year and Date: 20100606-20100610
• [Presentation] SUMO in plant development (2010)
  • Author(s): 石田喬志
  • Organizer: 第8回SUMO研究会
  • Place of Presentation: つくば市 日本
  • Year and Date: 2010-11-26
• [Presentation] SUMO in plant development (2010)
  • Author(s): 石田喬志
  • Organizer: 植物科学若手研究会2010
  • Place of Presentation: 神戸市 日本
  • Year and Date: 2010-10-23
• [Presentation] SUMO in plant development (2010)
  • Author(s): 石田喬志
  • Organizer: RIKEN PSC retreat
  • Place of Presentation: 横浜市 日本
  • Year and Date: 2010-10-12
• [Presentation] オーキシン依存的な細胞分裂周期と核内倍加周期の切り替えによる細胞分化制御 (2010)
  • Author(s): 石田喬志,安達澄子,吉村美香,清水皓平,梅田正明,杉本慶子
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 春日井市 日本
  • Year and Date: 2010-09-11
• [Presentation] SUMO E3 Ligase HIGH PLOIDY2 Regulates Endocycle Onset and Meristem Maintenance in Arabidopsis (2010)
  • Author(s): 石田喬志,杉本慶子
  • Organizer: 第51回日本植物生理学年会
  • Place of Presentation: 熊本市 日本
  • Year and Date: 2010-03-21
• [Presentation] オーキシン依存的な細胞分裂周期と核内倍加周期の切り替えによる細胞分化制御 (2010)
  • Author(s): 石田喬志,安達澄子,吉村美香,清水皓平,梅田正明,杉本慶子
  • Organizer: 第51回日本植物生理学年会
  • Place of Presentation: 熊本市 日本
  • Year and Date: 2010-03-20
• [Presentation] HPY2は核内倍加周期への移行とメリステム維持の制御に関わる新規SUMO E3 ligaseである (2010)
  • Author(s): 石田喬志,藤原すみれ,三浦謙治,Stacey Nicola,吉村美香,Schneider Katja,安達澄子,南澤一徳,梅田正明,杉本慶子
  • Organizer: 第51回日本植物生理学年会
  • Place of Presentation: 熊本市 日本
  • Year and Date: 2010-03-19
• [Presentation] オーキシンに応答したCDKB2の発現制御機構の解析 (2010)
  • Author(s): 清水皓平,石田喬志,杉本慶子,奥島葉子,梅田正明
  • Organizer: 第51回日本植物生理学年会
  • Place of Presentation: 熊本市 日本
  • Year and Date: 2010-03-19
• [Presentation] DEVELOPMENTAL CONTROL OF CELL SIZE AND PLOIDY IN ARABIDOPSIS (2009)
  • Author(s): 石田喬志
  • Organizer: 9th IPMB Congress 2009
  • Place of Presentation: St.Louis USA
  • Year and Date: 20091025-20091030
• [Presentation] SUMO E3 Ligase HIGH PLOIDY2/AtMMS21 Regulates Endocycle Onset and Stem Cell Maintenance in Arabidopsis (2009)
  • Author(s): 石田喬志,藤原すみれ,Stacey Nicola,吉村美香,Schneider Katja,杉本慶子
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜 日本
  • Year and Date: 2009-12-12
• [Presentation] SUMO E3 Ligase HIGH PLOIDY2 Regulates Endocycle Onset and Meristem Maintenance in Arabidopsis (2009)
  • Author(s): 石田喬志
  • Organizer: 特定領域研究「植物メリステム」若手ワークショップ2009
  • Place of Presentation: 千歳市 日本
  • Year and Date: 2009-10-08
• [Presentation] 細胞増殖と核内倍加を制御する分子機構の解析 (2009)
  • Author(s): 石田喬志,藤原すみれ,Schneider Katja,Stacey Nicola,杉本慶子
  • Organizer: 日本植物学会第73回大会
  • Place of Presentation: 山形市 日本
  • Year and Date: 2009-09-19
• [Presentation] 細胞増殖と核内倍加を制御する分子機構の解析 (2009)
  • Author(s): 石田喬志
  • Organizer: 細胞周期合同セミナー(2009年度)
  • Place of Presentation: 東京 日本
  • Year and Date: 2009-06-26