2010 Fiscal Year Final Research Report
Visuallization of the spatio-temporal activities of Rab GTPases forming Rab cascades
| Project/Area Number |
21870050
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
SUMIYA Nobuko The Institute of Physical and Chemical Research, 中野生体膜研究室, 特別研究員 (80534601)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 小胞輸送 / 出芽酵母 / 分泌小胞 / Rab GTPase / FRET |
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| Research Abstract |
Rab GTPase cascade in the regulation of ER-to-Golgi step of the secretory pathway was proposed in Saccaromyces cerevisiae. Ypt1p involved to ER to cis-Golgi transport acts upstream of the Ypt31/32p, mediating the budding of post-Golgi vesicles from the trans-Golgi. Ypt31/32p acts upstream of Sec4p, the Rab GTPase associated with post Golgi derived secretory vesicles. In this study, I developed the intra molecular FRET probes of these Rab GTPases to visualize the spacio-temporal activities in the living yeast cells.
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