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2010 Fiscal Year Final Research Report

Isolation and Evolutionary Ecological Analysis of the "Speciation Gene" of the genus Mitella

Research Project

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Project/Area Number 21870051
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Evolutionary biology
Research InstitutionNational Museum of Nature and Science, Tokyo

Principal Investigator

OKUYAMA Yudai  National Museum of Nature and Science, Tokyo, 植物研究部, 研究員 (40522529)

Project Period (FY) 2009 – 2010
Keywords種分化 / SuperSAGE法 / トランスクリプトーム / 次世代DNAシーケンサー / 遺伝マーカー / 花香成分
Research Abstract

I conducted a transcriptome analysis of the scent-emitting flowers of 10 Asimitellaria species, which comprises the vast majority of the section, using SuperSAGE. I analyzed the obtained SuperSAGE tags using perl-baseed bioinformatics and succeeded the genome-wide comprehensive transcriptome comparisons among closely related plant species. Further analyses based on gene expression and sequence data revealed some candidate genes dominating pollination systems of Asimitellaria, which were thus likely to be the "speciation genes".

  • Research Products

    (16 results)

All 2011 2010 2009 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (8 results) Book (2 results) Remarks (2 results)

  • [Journal Article] A multi-faceted genomics approach allows the isolation of rice Pia-blast resistance gene consisting of two adjacent NBS-LRR protein genes.2010

    • Author(s)
      Yudai Okuyama, Hiroyuki Kanzaki, Akira Abe, Kentaro Yoshida, Muluneh Tamiru, Hiromasa Saitoh, Takahiro Fujibe, Hideo Matsumura, Matt Shenton, Dominique Clark Galam, Jerwin Undan, Akiko Ito, Teruo Sone, Ryohei Terauchi
    • Journal Title

      The Plant Journal 66

      Pages: 467-479

    • Peer Reviewed
  • [Journal Article] Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development.2010

    • Author(s)
      Makoto Shirakawa, Haruko Ueda, Tomoo Shimada, Yasuko Koumoto, Takashi L., Shimada, Maki Kondo, Taku Takahashi, Yudai Okuyama, Mikio Nishimura, Ikuko Hara-Nishimura
    • Journal Title

      The Plant Journal 64

      Pages: 924-935

    • Peer Reviewed
  • [Journal Article] Which genus to study? In search of plant genera underrepresented or overrepresented in the research from the flora of Japan.2010

    • Author(s)
      Yudai Okuyama
    • Journal Title

      Bulletin of the National Museum of Nature and Science. Series B, Botany 36

      Pages: 82-89

    • Peer Reviewed
  • [Journal Article] Unveiling cryptic species diversity of flowering plants : successful biological species identification of Asian Mitella using nuclear ribosomal DNA sequences.2009

    • Author(s)
      Yudai Okuyama, Makoto Kato
    • Journal Title

      BMC Evolutionary Biology 9

      Pages: 105

    • Peer Reviewed
  • [Presentation] 形質、遺伝子、適応をつなぐ-新しい多様性生物学への挑戦-2011

    • Author(s)
      奥山雄大
    • Organizer
      日本生態学会第58回全国大会
    • Place of Presentation
      札幌市札幌コンベンションセンター
    • Year and Date
      2011-03-10
  • [Presentation] 多様な生活史形質を決定する遺伝子の探索:非モデル生物で行う多種間比較オミクス2011

    • Author(s)
      奥山雄大
    • Organizer
      日本分類学会連合第10回公開シンポジウム
    • Place of Presentation
      国立科学博物館新宿分館
    • Year and Date
      2011-01-09
  • [Presentation] Use of phylogenetic-based multi-species comparison to identify the key determinants in plant-pollinator interactions.2010

    • Author(s)
      Yudai Okuyama, Tomoko Okamoto, Ryutaro Goto, Makoto Kato
    • Organizer
      Memorial Symposium for the 26th International Prize for Biology "Biology of Symbiosis" -Celebrating Dr. Nancy A. Moran-
    • Place of Presentation
      茨城県つくば市エポカルつくば
    • Year and Date
      2010-12-07
  • [Presentation] 分子系統樹を用いた比較法と祖先形質復元:膨大な生物多様性情報を活用するために 進化学・夏の学校2010

    • Author(s)
      奥山雄大
    • Organizer
      日本進化学会第12回東京大会
    • Place of Presentation
      東京工業大学大岡山キャンパス
    • Year and Date
      2010-08-02
  • [Presentation] ゲノム時代の多様性生物学:チャルメルソウ属の進化・自然史研究からの展望2010

    • Author(s)
      奥山雄大
    • Organizer
      第35回駒場進化セミナー
    • Place of Presentation
      東京大学駒場キャンパス
    • Year and Date
      2010-02-22
  • [Presentation] モデル生物からの脱却:次世代シーケンサー/SuperSAGE法の応用によるゲノム系統学の試み2009

    • Author(s)
      奥山雄大
    • Organizer
      第41回種生物シンポジウム
    • Place of Presentation
      八王子セミナーハウス
    • Year and Date
      2009-12-12
  • [Presentation] Speciation at a single chemical : an extensive multi-species comparison revealed a key floral volatile for pollinator isolation in Asian Mitella.2009

    • Author(s)
      奥山雄大
    • Organizer
      National Museum of Nature and Science, International Symposium 2009.
    • Place of Presentation
      国立科学博物館新宿分館
    • Year and Date
      2009-11-22
  • [Presentation] Phylogenetic dissection of pollination syndrome of floral volatiles in Asian Mitella.2009

    • Author(s)
      Yudai Okuyama, Tomoko Okamoto, Makoto Kato
    • Organizer
      Evolution 2009 American Society of Naturalists, Society for the Study of Evolution, and Society of Systematic Biologists, Annual Meetings.
    • Place of Presentation
      University of Idaho, Moscow, ID USA
    • Year and Date
      2009-06-15
  • [Book] 日本の固有植物(加藤雅啓、海老原淳編)2010

    • Author(s)
      奥山雄大, 他共著
    • Total Pages
      503
    • Publisher
      東海大学出版会
  • [Book] The Handbook of Plant Mutation Screening : Mining of Natural and Induced Alleles. Chapter 11. Use of TILLING for Reverse and Forward Genetics of Rice.2010

    • Author(s)
      Sujay Rakshit, Hiroyuki Kanzaki, Hideo Matsumura, Arunita Rakshit, Takahiro Fujibe, Yudai Okuyama, Kentaro Yoshida, Muluneh Oli, Matt Shenton, Hiroe Utsushi, Chikako Mitsuoka, Akira Abe, Yutaka Kiuchi, Ryohei Terauchi.
    • Total Pages
      460(185-197)
    • Publisher
      Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany
  • [Remarks] ホームページ等

    • URL

      http://www.kahaku.go.jp/research/researcher/researcher.php?d=yokuyama

  • [Remarks]

    • URL

      https://sites.google.com/site/okuyamanokenkyuupeji/home

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Published: 2012-01-26   Modified: 2016-04-21  

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