Mechanism regulating sex determination of the germline progenitors in Drosophila

2010 Fiscal Year Final Research Report

• Project/Area Number: 21870054
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設) (2010); National Institutes of Natural Sciences Okazaki Research Facilities (2009)
• Principal Investigator: HASHIYAMA Kazuya 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 特別協力研究員 (20546949)
• Project Period (FY): 2009 – 2010
• Keywords: ショウジョウバエ / 生殖細胞 / 性決定

Research Abstract

Germline sex is imposed by surrounding soma after gonad formation. Here, we found that Sex lethal (Sxl) gene is expressed in the migrating PGCs in a female (XX) specific manner. To examine whether Sxl is able to induce female fate in the male (XY) PGCs, we expressed Sxl in XY PGCs. Then we transplanted the XY PGCs into normal female embryos and examined their fate. The feminized germline contributed progeny production. This suggests that Sxl is both required and sufficient for the female fate in the germline.

Research Products

• [Presentation] Sex lethal acts autonomously in the primordial germ cells to initiate female fate in Drosophila. (2011)
  • Author(s): Kazuya Hashiyama, Yoshiki Hayashi, Satoru Kobayashi.
  • Organizer: IRB-Barcelona, Barcelona, Spain.
  • Place of Presentation: Invited seminar
  • Year and Date: 2011-04-21
• [Presentation] Sex lethal acts autonomously in the primordial germ cells to initiate female fate in Drosophila. (2011)
  • Author(s): Kazuya Hashiyama, Yoshiki Hayashi, Satoru Kobayashi.
  • Organizer: Heinrich-Heine Universitat, Dusseldorf, Germany.
  • Place of Presentation: Invited seminar
  • Year and Date: 2011-03-28
• [Presentation] Sex lethal acts autonomously in the primordial germ cells to initiate female fate in Drosophila. (2011)
  • Author(s): Kazuya Hashiyama, Yoshiki Hayashi, Satoru Kobayashi.
  • Organizer: JSDB/Gfe Joint meeting, Dresden, Germany.
  • Place of Presentation: Oral presentation
  • Year and Date: 2011-03-24
• [Presentation] Sex lethal acts autonomously in the germline progenitors to initiate female development in Drosophila. (2010)
  • Author(s): Kazuya Hashiyama, Yoshiki Hayashi, Satoru Kobayashi.
  • Organizer: Carnegie institution, Baltimore, U.S.A.
  • Place of Presentation: Oral presentation
  • Year and Date: 2010-10-14
• [Presentation] Sex lethal acts autonomously in the germline progenitors to initiate female development in Drosophila. (2010)
  • Author(s): Kazuya Hashiyama, Yoshiki Hayashi, Satoru Kobayashi.
  • Organizer: Cold Spring Harbor Laboratory Meeting, New York, U.S.A.
  • Place of Presentation: Poster presentation
  • Year and Date: 2010-10-06
• [Presentation] Mechanism regulating sex determination of the germline progenitors in Drosophila. (2010)
  • Author(s): Kazuya Hashiyama, Yoshiki Hayashi, Satoru Kobayashi.
  • Organizer: Sattellite Symopium to SDB and JSDB joint meeting, Albuqurque, U.S.A.
  • Place of Presentation: Oral presentation
  • Year and Date: 2010-08-05