2010 Fiscal Year Final Research Report

Peptidome analysis focusing on C-terminal amidation : Identification and functional analysis of novel bioactive peptides.

Research Project

Project/Area Number	21870055
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Functional biochemistry
Research Institution	National Cardiovascular Center Research Institute
Principal Investigator	WAKABAYASHI Masaki 独立行政法人国立循環器病研究センター, 分子薬理部, 研究員 (70552024)
Project Period (FY)	2009 – 2010
Keywords	生理活性ペプチド / ペプチドーム解析 / C末端アミド化 / 抗体アフィニティーカラム
Research Abstract	Bioactive peptides play crucial roles in complex homeostasis regulatory systems in living organisms. Thus, it is essential to identify novel bioactive peptides and analyze their physiological functions to elucidate the homeostasis regulatory systems. In this study, we tried to isolate new bioactive peptides using their frequently observed structural characteristic, C-terminal amidation, as a hallmark. We developed a method for condensation of C-terminally amidated peptides from naturally occurring peptide mixtures, though a novel bioactive peptide was not identified.

(1 results)