Identification of the regulatory factor for insect-form transformation of trypanosomes from tsetse flies

2010 Fiscal Year Final Research Report

• Project/Area Number: 21880003
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Hokkaido University
• Principal Investigator: MIYAZAKI Satoshi Hokkaido University, 大学院・獣医学研究科, 博士研究員 (20547781)
• Project Period (FY): 2009 – 2010
• Keywords: アフリカ睡眠病 / トリパノソーマ / ツェツェバエ / 分化制御因子 / サシバエ / 遺伝子重複 / RACE法 / セリンプロテアーゼ

Research Abstract

To control the African Sleeping Sickness, which is caused by the infection of trypanosome parasites (Trypanosoma spp.) into mammalian blood, it is required to understand the regulatory mechanisms for cell differentiation into insect form parasite at the timing of infection. In this study, the full length of Glossina serine protease 1 (Gsp1) was isolated by RACE PCR as the candidate of regulatory molecules, and it was showed that Gsp1 have the trypsin-like serine protease domain. Considering the previous studies, one of the serine protease genes duplicated might gain the novel function for controlling the insect-form transformation of Trypanosoma. I

Research Products

• [Journal Article] Ovarian development and insulin-signaling pathways during reproductive differentiation in the queenless ponerine ant Diacamma sp. (2010)
  • Author(s): Y.Okada, S.Miyazaki, H.Miyakawa, A.Ishikawa, K.Tsuji, T.Miura
  • Journal Title: Journal of Insect Physiology 56 Pages: 288-295
  • Peer Reviewed
• [Journal Article] Evaluation of Myanmar medicinal plant extracts for antitrypanosomal and cytotoxic activities. (2010)
  • Author(s): S.Bawn, S.Tiwananthagorn, K.S.Lin, J.Hirota, T.Irie, L.L.Htun, N.N.Maw, T.T.Myaing, N.Phay, S.Miyazaki, T.Sakurai, Y.Oku, H.Matsuura, K.Katakura
  • Journal Title: The Journal of Veterinary Medical Science 72 Pages: 525-528
  • Peer Reviewed
• [Journal Article] Ergatoid queen development in the ant Myrmecina nipponica : modular and heterochronic regulation of caste differentiation. (2010)
  • Author(s): S.Miyazaki, T.Murakami, T.Kubo, N.Azuma, S.Higashi, T.Miura
  • Journal Title: Proceedings of the Royal Society B 277(1690) Pages: 1953-1961
  • Peer Reviewed
• [Journal Article] Gene up-regulation in response to predator kairomones in the water flea, Daphnia pulex. (2010)
  • Author(s): H.Miyakawa, M.Imai, N.Sugimoto, Y.Ishikawa, A.Ishikawa, H.Ishigaki, Y.Okada, Miyazaki, S., S.Koshikawa, R.Cornette, T.Miura
  • Journal Title: BMC Developmental Biology 10 Pages: 45, doi:10.1186/1471-213X-10-45
  • Peer Reviewed
• [Journal Article] Identification of a reproductive-specific, putative lipid transport protein gene in a queenless ponerine ant Diacamma sp. (2010)
  • Author(s): Y.Okada, S.Miyazaki, S.Koshikawa, R.Cornette, K.Maekawa, K.Tsuji, T.Miura
  • Journal Title: Naturwissenschaften. 97 Pages: 971-979
  • Peer Reviewed
• [Presentation] 宿主ー媒介昆虫間の移動に伴うトリパノソーマ原虫Trypanosoma bruceiの表現型分化における分子制御機構 (2011)
  • Author(s): 宮崎智史,櫻井達也,片倉賢
  • Organizer: 日本生態学会第58回大会
  • Place of Presentation: 札幌コンベンションセンター 札幌
  • Year and Date: 2011-03-11
• [Presentation] Isolation and characterization of serine proteases of tsetse and stable flies, and their roles in the differentiation of bloodstream forms of Trypanosoma brucei into procyclic forms. (2010)
  • Author(s): Miyazaki S., Sakurai T., Oku Y., Katakura K.
  • Organizer: The 2nd International Young Researcher Seminar in Zoonosis Control
  • Place of Presentation: Hokkaido University, Sapporo
  • Year and Date: 2010-09-13
• [Presentation] Ergatoid queen differentiation in the ant Myrmecina nipponica: Modular and heterochronic regulation of caste differentiation. (2010)
  • Author(s): T.Miura, Miyazaki S.
  • Organizer: The 49th annual Canadian Society of Zoologists Meeting
  • Place of Presentation: University of British Coloumbia, Vancouver, Canada
  • Year and Date: 2010-05-18
• [Presentation] トゲオオハリアリの体色にみられる性的二型の発現機構 (2009)
  • Author(s): 宮崎智史
  • Organizer: 京都大学生態学研究センター共同利用事業 研究集会 「表現型可塑性の生物学」
  • Place of Presentation: 北海道大学 札幌
  • Year and Date: 2009-11-14
• [Presentation] トゲオオハリアリDiacamma sp.(Hymenoptera : Formicidae)にみられる顕著な性的二型発現の分子制御機構 (2009)
  • Author(s): 宮崎智史,三浦徹
  • Organizer: 第11回日本進化学会大会, P01-02, ポスター発表
  • Place of Presentation: 北海道大学 札幌
  • Year and Date: 2009-09-03
• [Presentation] Developmental mechanisms of distinct sexual dimorphism in the ponerine ant Diacamma sp.(Hymenoptera : Formicidae). (2009)
  • Author(s): S.Miyazaki, K.Katakura, Y.Oku, H.Gotoh, H.Miyakawa, T.Miura
  • Organizer: The 1st International Young Researcher Seminar in Zoonosis Control 2009, P-9, Poster Presentation
  • Place of Presentation: Venue Hotel Niseko Ikoinomura
  • Year and Date: 2009-08-25