2010 Fiscal Year Final Research Report
Study of substrate transport mechanism of spermidine exporter by means of proteoliposome
| Project/Area Number |
21880011
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Tohoku University |
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Principal Investigator |
NANATANI Kei 東北大学, 大学院・工学研究科, 助教 (00547333)
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| Project Period (FY) |
2009 – 2010
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| Keywords | ポリアミン / トランスポーター / 単分子FRET / リポソーム / 塩ストレス適応 |
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| Research Abstract |
The purpose of this study is to understand the relationship of intracellular polyamine and osmotic stress adaptation in bacterial cells. We investigated the polyamine transport mechanism of the ABC transporter by using the single molecule FRET technology. In addition, we reveal the contribution of polyamine synthetic enzymes (ADC1 and ADC2)to biofilm formation in Synechocystis sp. PCC 6803 under salt stress condition by means of adc1 and adc2 gene knockout mutants.
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