2010 Fiscal Year Final Research Report
Study of a new transgenic technique in coenocytic green algae utilizing their protoplast regeneration ability
| Project/Area Number |
21880029
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
General fisheries
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| Research Institution | Hiroshima University |
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Principal Investigator |
HIRAYAMA Makoto Hiroshima University, 大学院・生物圏科学研究科, 助教 (40535465)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 遺伝子改変技術 / 海藻 / 細胞再構築現象 / ハネモ |
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| Research Abstract |
Reporter mRNA encoding Renilla luciferase was introduced into the sub-protoplasts, which were regenerated by squeezing out from the coenocytic green alga Bryopsis plumosa, using conventional transgenic methods with polyethyleneglycol and cationic lipid or a new method of which the reporter mRNA was added into the squeezing medium. The luciferase activities were detected in those sub-protoplasts introduced by the new method as well as by the conventional methods. These results indicated that a new easy transgenic technique in coenocytic green algae could be established.
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