2010 Fiscal Year Final Research Report
Analysis of ER associated degradation from structure of sugar chain in Aspergillus oryzae
| Project/Area Number |
21880055
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
KIKUMA Takashi The Institute of Physical and Chemical Research, 伊藤細胞制御化学研究室, 特別研究員 (90553842)
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| Project Period (FY) |
2009 – 2010
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| Keywords | 麹菌 / 小胞体 / 糖鎖 / 小胞体関連分解 |
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| Research Abstract |
Yeast Htm1 is a mannosidase-like protein related to ERAD. We obtained strains that overexpress the Htm1 homologue in A. oryzae, Aohtm1. Trimming of the B arm of Man9GlcNac2-PA to Man8Glc2-PA was accelerated in that strain, indicating AoHtm1 was involved in processing of N-linked oligosaccharide. Furthermore, recombinant AoHtm1 expressed in E. coli has mannosidase activity. This suggests the possibility that AoHtm1 is a novel a-1,2 mannosidase.
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